| 镉诱导小鼠胸腺细胞凋亡的研究 |
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| 引用本文： | 董书芸,沈汉明,王俊南.镉诱导小鼠胸腺细胞凋亡的研究[J].中国职业医学,2000,27(6):9-11. |
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| 作者姓名： | 董书芸 沈汉明 王俊南 |
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| 作者单位： | 1. 中山医科大学公共卫生学院，广东广州 510080
2. 新加坡国立大学社区、职业与家庭医学系 |
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| 基金项目： | 中华医学基金会(CMB) |
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| 摘 要： | 目的 探索镉的免疫毒性机制，系统性地研究镉体外诱导小鼠胸腺细胞凋亡的发生，解释其细胞毒性机制。方法 通过以下方法检测凋亡；末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL），DNA含量/细胞周期分析，DNA琼脂糖凝胶电泳。结果 镉能引起原代培养的胸腺细胞发生凋亡，并呈时间-反应和剂量-效应关系。结论 本研究为阐述镉免疫毒性机制提供了依据，尤其对解释镉引起的胸腺萎缩和导致的T细胞介导的免疫力损伤有重要意义。
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| 关 键 词： | 镉 胸腺细胞 细胞凋亡 TUNEL 免疫毒性 |
| 文章编号： | 1000-6486(2000)06-0009-03 |
Studies on cadmium-induced apoptosis in mouse thymocytes |
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| ONG Shu-yun,SHEN Han-ming,Ong Choon-Nam.Studies on cadmium-induced apoptosis in mouse thymocytes[J].China Occupational Medicine,2000,27(6):9-11. |
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| Authors: | ONG Shu-yun SHEN Han-ming Ong Choon-Nam |
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| Abstract: | Objective To elucidate the immunotoxical mechanism of cadmium (Cd) which is a common occupational and environmental contaminant, apoptical effect of Cd on mouse thymoytes was studied. Methods The Cd-induced thymocyte apoptosis was systematically determined by ①TdT-mediated dUTP nick end labeling (TUNEL) assay; ②DNA content/cell cycle analysis; ③DNA agarose gel electrophoresis. Results The results showed that Cd might cause time-and dose-dependent apoptosis in primary cultured thymocytes. Conclusion These findings would be of significance in the understanding of immunotoxic mechanism of Cd, especially the induction of thymus atrophy and T cell mediated immunity. |
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| Keywords: | Cadmium apoptosis thymocyte TUNEL |
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