CD14基因-260A/G位点多态性与前列腺癌易感性的研究

目的研究CD14基因-260A/G位点多态性与前列腺癌易感性的关系。方法提取168例前列腺癌患者和208例对照组的外周血DNA标本,应用聚合酶链反应-连接酶特异检测技术（polymerase chain reaction-ligase detection reaction,PCR-LDR）分析前列腺癌患者CD14-260A/G位点多态性,比较不同基因型和前列腺癌易感性的关系,并探讨不同基因型与前列腺癌患者年龄、体重指数（body mass index,BMI）、吸烟、饮酒及肿瘤家族史的关系。结果总体来说CD14基因-260A/G多态性与前列腺癌易感性之间无显著相关性（P=0.284,OR=1.27,95%CI=0.82～1.94）;饮酒（P=0.003）和肿瘤家族史（P〈0.001）与前列腺癌的发生密切相关;在分层分析中,年龄〈70岁的男性携带CD14-260G（AG＋GG）等位基因者能增加前列腺癌的易感性（P=0.011,OR=2.93,95%CI=1.28～6.70）。结论 CD14基因-260A/G位点多态性在总体上与前列腺癌易感性无显著相关性,但在年龄小于70岁男性中,携带有CD14-260G等位基因者患前列腺癌的危险性却明显增高。

Association between CD14-260 polymorphism and the risk of the prostate cancer

Objective To investigate the association between polymorphism in CD14-260A/G and the risk of prostate cancer.Methods The polymorphism of CD14-260A/G was analyzed with polymerase chain reaction-ligase detection reaction(PCR-LDR) technique using genomic DNA isolated from peripheral blood.Moreover,the relation of prostate cancer with patients’ age,body mass index(BMI),smoking and drinking habit,and family history in different genotypes.Results Overall,CD14-260 gene A/G polymorphism didn’t increase the risk of prostate cancer(P=0.284,OR=1.27,95% CI=0.82-1.94).In the stratified analysis,drinking habit(P<0.003) and family history of cancer(P<0.001) had a significantly increased association with prostate cancer.In addition,CD14-260 G(AG+GG) allele increased the risk of prostate cancer only in patients younger than 70(P=0.011,OR=2.93,95%CI=1.28-6.70).Conclusions CD14-260 A/G polymorphism had no association with the risk of prostate cancer.However,CD14-260 G(AG+GG) allele could increase the risk of prostate cancer in patients younger than 70 years of age.