金纳米链及抗表皮生长因子受体抗体/金纳米链共轭物近红外热疗可启动细胞凋亡通路

背景:金纳米颗粒在近红外激光照射下对肿瘤细胞具有杀伤效应。目的:评价金纳米链及抗表皮生长因子受体抗体/金纳米链共轭物近红外热疗对人喉癌Hep-2细胞的热杀伤作用并探讨其凋亡通路。方法:取生长良好的人喉鳞癌Hep-2细胞,分别应用金纳米链或抗表皮生长因子受体抗体/金纳米链共轭物溶液进行干预,在此基础上应用8W/cm2近红外激光(808nm)照射6min,以单独应用1640培养液培养及单独给予近红外激光照射的细胞作对照。结果与结论:透射电镜观察发现,金纳米链热疗后Hep-2细胞有明显的损伤,抗表皮生长因子受体抗体/金纳米链热疗后细胞的损伤程度最重。Westernblot检测显示,金纳米链热疗和抗表皮生长因子受体抗体/金纳米链热疗后细胞热休克蛋白70和促凋亡蛋白Bax表达明显增高,而抑凋亡蛋白Bcl-2表达明显降低。说明金纳米链及抗表皮生长因子受体抗体/金纳米链共轭物近红外热疗可通过启动细胞凋亡通路杀伤人喉癌Hep-2细胞。

Gold nanochain and anti-epidermal growth factor receptor/Au near-infrared hyperthermia can start apoptosis pathway

BACKGROUND: Gold nanoparticles have a killing effect on tumor cells under the near-infrared laser irradiation. OBJECTIVE: To evaluate the killing effect of gold nanochain and anti-epidermal growth factor receptor (EGFR)/Au conjugates in near-infrared hyperthermia on human laryngeal cancer Hep-2 cells and to explore relevant apoptosis pathway. METHODS: Human laryngeal squamous carcinoma Hep-2 cells were extracted and intervened in gold nanochain or anti-EGFR/Au solution. Then, the Hep-2 cells were exposed to the near-infrared laser irradiation of 8 W/cm2 (808 nm) for 6 minutes. The cells cultured alone in 1640 culture medium or only exposed to near-infrared laser were as controls. RESULTS AND CONCLUSION: Under transmission electron microscope, the Hep-2 cells cultured in the gold nanochain solution after hyperthermia were significantly damaged, and the cells in the anti-EGFR/Au solution after hyperthermia were damaged worst. The Western blot detection exhibited that the expressions of heat-shock protein 70 and pro-apoptotic protein Bax were significantly increased, while the expression of anti-apoptotic protein Bcl-2 was obviously decreased exposed to gold nanochain and anti-EGFR/Au near-infrared hyperthermia. These suggest that gold nanochain and anti-EGFR/Au near-infrared hyperthermia can kill human laryngeal cancer Hep-2 cells via apo-ptosis pathway activation.