蓝舌病毒湖北株对人前列腺癌PC-3细胞杀伤效应的研究

目的体外实验研究蓝舌病毒湖北株3（BTV-HbC3）对人前列腺癌PC-3细胞的杀伤效应并且初步探讨BTV-HbC3靶向性溶瘤的机制。方法观察PC-3细胞感染BTV-HbC3后所致的病变效应（CPE）变化;透射电镜观察细胞感染病毒后超微结构的变化;CCK-8法研究病毒致细胞病变效应的特征;DNA Ladder分析病毒诱导细胞凋亡的情况;流式细胞仪检测病毒对PC-3细胞凋亡的影响。结果 BTV-HbC3感染PC-3细胞后可见到明显的细胞效应;透射电镜发现胞浆内有大量病毒颗粒和典型细胞凋亡的形态变化;CCK-8法检测到病毒明显抑制细胞生长;DNA Ladder分析见阶梯状条带;流式细胞仪检测到明显细胞凋亡的存在,可见明显的亚二倍体峰。结论 BTV-HbC3在体外可有效地杀伤PC-3细胞,并能诱导其凋亡。

The lethal effect of bluetongue virus strain HbC3 on PC-3 cells

Objective To investigate the characteristics and the mechanism of bluetongue virus strain HbC3(BTV-HbC3) infecting PC-3 malignancy in vitro.Methods PC-3 cells were infected with BTV-HbC3 to observe the cytopathic effects(CPE).Changes of ultrastructure of the infected cells were observed with transmission electron microscope(TEM).The inhibition activity of the infected PC-3 cells was analyzed with CCK-8.The apoptosis of PC-3 cells induced by BTV-HbC3 was detected with DNA ladder.The effect of virus on the apoptosis of PC-3 cells was detected by flow cytometry(FCM).Results Obvious CPE was observed.TEM observation found lots of virus particles in the cytoplasm and typical changes of ultrastructure.CCK-8 detected that the virus could significantly inhibit the growth of cells.DNA ladder found ladder strip shape.FCM detected significant cell apoptosis with hypodiploid peak.Conclusions BTV-HbC3 is able to infect and replicate in PC-3 cells effectively in vitro and induce tumor cell apoptosis.