体外诱导大鼠骨髓间充质干细胞向神经元样细胞的分化(英文)

背景:研究表明,移植后的骨髓间充质干细胞在新的环境中能够被诱导分化为神经元样细胞,从而替代损伤细胞重建神经环路。目的:建立大鼠骨髓间充质干细胞和神经细胞的体外共培养系统,观察共培养条件下神经细胞对骨髓间充质干细胞向神经元样细胞分化的影响。方法:体外培养Wistar大鼠脑组织神经细胞和股骨骨髓间充质干细胞,采用Transwell小室共培养分化诱导,观察骨髓间充质干细胞的组织形态变化,在共培养第5天免疫荧光染色检测骨髓间充质干细胞中神经细胞的特异标志物;与对照组单纯骨髓间充质干细胞培养结果相比较。结果与结论:神经细胞共培养系统中的骨髓间充质干细胞生长伸展,呈放射状,互相形成连接,特异性烯醇化酶显示阳性结果,具有神经元样细胞的特性,表达特异性烯醇化酶阳性的神经元比例可达(33.0±10.5)%。而对照组骨髓间充质干细胞未形成神经元样细胞的形态结构,免疫荧光显示特异性烯醇化酶阴性。提示神经细胞提供的微环境对骨髓间充质干细胞分化为神经元样细胞具有诱导分化作用。

Rat bone marrow mesenchymal stem cells differentiation into neuron-like cells in vitro

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can be induced to differentiate into neuron-like cells in new environment after transplantation, and then to replace damaged cells for reconstructing neural circuit. OBJECTIVE: To establish the co-culture system between rat BMSCs and neural cells in vitro, and to study the influence of neural cells on the differentiation of BMSCs into neuron-like cells in the co-culture system. METHODS: The neural cells obtained from Wistar rat fetal brain tissue and BMSCs gained from rat thighbone were co-cultured in Transwell culture plate. The morphological changes of BMSCs were observed and the special markers of neural cells in BMSCs were examined by immunofluorescence on the fifth day of the co-culture. The results were compared with control group which where BMSCs were alone cultured. RESULTS AND CONCLUSION: BMSCs in the neural cells co-culture system extended, were radial, connected each other. Neuron specific enolase immunoreactions showed positive results, showing neuron-like cells. The positive ratio of neuron specific enolase-positive cells was (33.0±10.5)%. However, BMSCs in the control group did not express neuron morphological character. Immunofluorescence exhibited that cells were negative for neuron specific enolase. These indicate that microenvironment provided by neurons improves the differentiation of BMSCs into neuron-like cells.