纯钛表面微形态可影响成骨细胞生长及β1整合素表达

背景:目前微弧氧化和渐热处理是常见的两种钛种植体表面化学成分改性方法。目的:观察纯钛表面微形态对成骨细胞生长的影响。方法:将原代培养的小鼠成骨细胞与不同表面处理的钛片:纯钛组、微弧氧化组、碱热组、微弧氧化碱热处理组共同培养。结果与结论:扫描电镜观察微弧氧化碱热处理组表面附着细胞呈扁平的多边形,且有更好的伸展,成骨细胞中β1整合素表达显著高于其他3组(P<0.05)。提示微弧氧化碱热处理组更有利于成骨细胞的黏附和β1整合素的表达。

Effect of titanium surface micromorphology on osteoblasts growth and beta1 integrin expression

BACKGROUND: Currently, micro-arc oxidation and gradually heat treatment are two common surface modification methods of chemical composition for titanium implant surfaces.OBJECTIVE: To study the effect of titanium surface micromorphology on growth of osteoblasts. METHODS: Primary cultured mouse osteoblasts were co-cultured with titanium plates with different surface treatments: titanium group, micro-arc oxidation group, alkali heat treatment group and micro-arc oxidation/alkali heat treatment group.RESULTS AND CONCLUSION: The cells attached to the surfaces in the micro-arc oxidation/alkali heat treatment group were flat polygons and had better stretch. Expression of β1 integrin in osteoblasts micro-arc oxidation/alkali heat treatment group was stronger than those of the other groups (P<0.05). The micro-arc oxidation combined with alkali heat treatment is more advantageous for the osteoblasts adhesion and expression of β1 integrin.