| 柯萨奇B3病毒对HL-1心肌细胞的影响 |
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| 引用本文： | 彭俊,钱正明,黄建振,高世龙.柯萨奇B3病毒对HL-1心肌细胞的影响[J].岭南心血管病杂志,2012,18(1):59-61,73. |
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| 作者姓名： | 彭俊 钱正明 黄建振 高世龙 |
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| 作者单位： | 杭州市萧山区第一人民医院心内科,杭州,311200 |
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| 摘 要： | 目的探讨柯萨奇B3病毒对HL-1心肌细胞影响。方法实时荧光聚合酶链反应检测柯萨奇B3病毒感染的HL-1心肌细胞Bcl-2及Bax的表达：Annexin V-PE凋亡检测试剂盒检测感染的HL-1心肌细胞发生凋亡的比率，并与未感染心肌细胞进行对比。结果感染后4h组（0．199±0．005vs0．104±0．014，P=0．0008）及24h组（0．154±0．005vs0．1044-0．014．P=0．0176）的Bel－2／Bax表达均显著高于未感染组，差异有统计学意义。感染后4h组的Bel-2／Bax表达高于感染后24h组，差异有统计学意义（0．199±0．005孤0．154±0．005，P=0．0009）。感染后4h组（9．160％±0．161％vs．0．908％±0．015％，P〈0．0001）、12h组（6．305％±0．231％vs．0．908％±0．015％．P〈0．0001）、24h组（3．260％±0．162％vs0．908％±0．015％，P〈0．0001）的心肌细胞凋亡率显著高于未感染组，差异均有统计学意义。感染后4h组的心肌细胞凋亡率高于感染后12h组（9．160％±0．161％vs．6．305％±0．231％．P〈0．0001）和24h组（9．160％±0．161％vs．3．260％±0．162％，P〈0．0001），差异均有统计学意义。结论柯萨奇B3病毒导致HL-1心肌细胞的凋亡，在感染4h后增加尤为明显。
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| 关 键 词： | 心肌炎 心肌细胞 柯萨奇B3病毒 凋亡 |
Effect of Coxsackievirus B3 on HL-1 myocardium cells |
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| PENG Jun
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QIAN Zheng-ming
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HUANG Jian-zhen
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GAO Shi-long.Effect of Coxsackievirus B3 on HL-1 myocardium cells[J].South China Journal of Cardiovascular Diseases,2012,18(1):59-61,73. |
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| Authors: | PENG Jun QIAN Zheng-ming HUANG Jian-zhen GAO Shi-long |
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| Institution: | (Department of Cardiology, The First People＇s Hospital of Xiaoshan, Hangzhou 311200, China) |
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| Abstract: | Objectives To investigate the effect of Coxsackievirus B3 on HL-1 myoeardium ceils. Methods We examined the expression of Bel-2 and Bax of infection HL-1 myoeardium cells by reai time polymerase chain reaction （PCR）, the apoptosis ratio by Annexin V-PE kit and compared them with those of non-infected HL-1 myocardium cells. Resuits Compared with non-infected group, Bcl-2/Bax of 4 h after infection group （0.199 ± 0.005 vs. 0.104 ± 0.014,P=0.0008） and 24 h after infection group （0.154 ± 0.005 vs. 0.104 ± 0.014,P=0.0176） increased significantiy. Compared with 24 h after infection group, Bcl-2/Bax of 4 h after infection group increased significantly （0.199 ± 0.005 vs. 0. 154 ± 0.005, P=0.0009）. Compared with non-infected group, apoptosis ration of 4 h after infection group （9.160% ±0.161% vs. 0.908% ±- 0.015%, P〈0.0001）, 12 h after infection group （6.305% ± 0.231% vs. 0.908% ± 0.015%, P〈0.0001） and 24 h after infection group （3.260% ±0.162% vs. 0.908% ± 0.015%, P〈0.0001） increased significantly. Compared with 12 h after infection group （9.160% ± 0.161% vs. 6.305% ±0.231%, P〈0.0001 ） and 24 h after infection group （9.160%±0.161% vs. 3.260%±0.162% ,P〈0.0001）, apoptosis ration of 4 h after infection group increased significantly. Conclusions Coxsackievirus B3 contributes to the apoptosis of HL-1 cells, especially 4 h after infection. Key words ： myocarditis ; myocardium cells ; Coxsackievirus B3 ; apoptosis |
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