氟处理AZ31B生物可降解镁合金材料的细胞相容性

背景:镁及镁合金作为新型可降解植入材料,具有优异的生物相容性和综合力学性能,但是由于镁及镁合金较差的耐蚀性能,影响到其在临床上的应用.表面氟化处理的AZ31B镁合金是否具有良好的生物相容性尚不清楚.目的:以诱导的人骨髓间充质干细胞作为检测细胞,评价不同表面氟处理的镁合金材料的细胞相容性.方法:以诱导的人骨髓间充质干细胞作为检测细胞,取未经氟处理及不同氟处理时间的镁合金材料浸提液进行体外细胞相容性实验,评价不同氟处理的镁合金AZ31B材料的生物相容性.结果与结论:经氟处理镁合金AZ31B材料与未经氟处理镁合金AZ31B材料相比能显著提高细胞存活率,细胞毒性分级1级,对细胞生长基本无毒性作用.提示经氟处理镁合金AZ31B材料生物相容性优于未经氟处理镁合金AZ31B材料.

Cellular compatibility of biodegradable AZ31B magnesium alloys with fluoride treatment

BACKGROUND: As a new type of biodegradable implant material, magnesium and its alloys have attracted much attention owing to their excellent biocompatibility and comprehensive mechanical properties. However, the clinical application of magnesium and its alloys is affected for their inferior corrosion. It is not confirmed whether AZ31B magnesium alloys with fluoride treatment can exhibit good biocompatibility. OBJECTIVE: To evaluate the cellular compatibility of magnesium alloy materials with different surface fluoride treatments. METHODS: The induced human bone marrow mesenchymal cells were taken as the testing cells. The leaching liquor of the magnesium alloy materials with no fluoride treatments and fluoride treatments in different time was extracted for cellular compatibility experiment in vitro. The biocompatibility of AZ31B magnesium alloy materials with different fluoride treatments was evaluated. RESULTS AND CONCLUSION: This experiment showed that compared with the AZ31B magnesium alloys without fluorine treatments, the cell survival rate of the AZ31B magnesium alloys with fluorine treatments was significantly increased; the toxicity of cells was in grade 1 and there was almost no toxicity to cell growth. These findings suggest that the biocompatibility of AZ31B magnesium alloys with fluoride treatments was superior to that of AZ31B magnesium alloys without fluorine treatments.