大鼠角膜碱烧伤后血小板源生长因子的表达

目的 :检测血小板源生长因子 (PDGF)在大鼠角膜碱烧伤后表达的变化情况。方法 :建立大鼠角膜碱烧伤模型 ,用免疫组化法检测角膜碱烧伤后不同时段PDGF BB在角膜各层次中的表达 ,用RT PCR法检测PDGF B的mRNA在角膜碱烧伤后不同时段表达的变化。结果 :免疫组化检测显示 ,在正常角膜中 ,PDGF BB在上皮层中为弱表达 ,角膜碱烧伤后 ,PDGF BB在角膜各层组织中均有表达且表达逐步增强。各实验组中 ,7d组表达最强 ,而后逐渐下降 ,至 30d组基质层已基本无表达。RT PCR检测显示 ,正常角膜和损伤角膜中均有PDGF B的mRNA表达 ,角膜碱烧伤后 7d组和15d组 ,PDGF B的mRNA表达量最多。结论 :PDGF B在角膜碱烧伤后表达增强 ,且其表达的量随病程的发展而变化 ,PDGF B在角膜的损伤和修复过程中起一定作用。

Expression of platelet-derived growth factor in rat cornea after cautery with alkali

Objective:To detect the gene expression of PDGF-B in the rat cornea after cautery with alkali.Methods:The corneas of Sprague Dawley rats were wounded by cautery with alkali. Immunohistochemistry and RT-PCR were used to detect PDGF-B in corneal specimens at different time periods.Results:Both immunohistochemistry and RT-PCR showed that the gene expression of PDGF-B is rare in normal corneas but its expression increased after alkali burn. Gene expression of PDGF-B is at the highest level approximately 7 days after corneal alkali burn.Conclusion:After corneal alkali burn,the gene expression of PDGF-B increased and may play an important role in corneal wound healing.