整合素α5和肌动蛋白在周期性压力下椎间盘细胞中的表达

目的 探讨整合素α5和肌动蛋白在周期性压力下椎间盘细胞中的表达。方法对猪椎间盘组织进行体外细胞的分离和培养,对细胞施加周期性液压,通过对细胞的形态学观察、Western免疫印迹和免疫组化染色,分别检测整合素α5和肌动蛋白在周期性压力下椎间盘细胞中的表达。结果经加压后,纤维环细胞和髓核细胞均可见体积缩小,由多角形转变为细长形,整合素α5和肌动蛋白在纤维环细胞和髓核细胞中的表达均明显减少。结论体外培养的单层椎间盘细胞在受力状态下,整合素α5的表达减少,整合素α5将力信号转换到细胞内时,进一步影响到与其关系密切的肌动蛋白。这可能是椎间盘细胞力学传导的重要机制。

Expression of integrin alpha5 and actin in the cells of intervertebral disc under cyclic hydrostatic pressure in vitro

OBJECTIVE: To investigate the expression of integrin alpha5 and actin in the cells of intervertebral disc under cyclic hydrostatic pressure in vitro. METHODS: The porcine lumbar intervertebral disc cells were isolated and cultured in vitro, and the cells underwent cyclic hydrostatic loading. After that, the expression of integrin alpha5 and actin in intervertebral disc cells were studied by means of morphology observing, Western blot and immunohistochemistry staining. RESULTS: The morphology of intervertebral disc cells were changed into smaller and flatten shape, and the expression of integrin alpha5 and actin were decreased after loading. CONCLUSIONS: The expression of integrin alpha5 decreases under cyclic hydrostatic pressure, and the actin is affected at the same time when signals are transferred into the cells by integrin alpha5. That may be one of the important mechanisms of the mechanotransduction in the cells of intervertebral disc.