慢性丙型肝炎病毒感染者甘露糖结合凝集素基因多态性研究

目的研究丙型肝炎病毒（HCV）感染者甘露糖结合凝集素（MBL）基因多态性。方法应用序列特异性引物PCR法（PCR-SSP）方法检测甘肃地区汉族150例慢性丙型肝炎及75例健康对照者MBL第一外显子（EXONⅠ）52、54、57位基因型。结果甘肃地区汉族人群MBL基因EXONⅠ的52,57位没有检测出突变体,而54位基因在HCV感染者和在正常对照者中的基因型频率和基因频率比较差异均无统计学意义（P〉0.05）;丙氨酸转氨酶（ALT）〉80U/L、ALT〈40U/L两组基因型频率和等位基因频率比较差异均有统计学意义（P〈0.05）;HCV-RNA定量中、高载量组与低载量组MBL-54位基因型频率和基因频率比较差异也均有统计学意义（P〈0.05）。结论 HCV感染者MBL的EXONⅠ54位基因多态性与慢性HCV感染无显著相关性,而54位等位基因与ALT的升高和HCV-RNA载量有一定的相关性。

Genetic association of mannan-binding lectin polymorphisms with hepatitis C virus infection

Objective To investigate the association between gene polymorphisms in mannan-binding lectin（MBL）and HCV infection.Methods 150 Han ethnic patients with chronic hepatitis C disease and 75healthy controls were studied.The polymorphisms were perfotmed by PCR-SSP.Results The difference in MBL gene genetype and allele distributions between patients with chronic HCV infection and controls was not significant.MBL gene was significantly different at position 54genotypes and allele distributions between ALT＆gt;80U/L than in those with ALT＆lt;80U/L groups（P＆lt;0.05）.MBL gene was significantly different at position 54genotypes and allele distributions between medium high and low HCV RNA loading groups.Conclusion The polymorphisms in MBL gene at position 54was not associated with HCV infection.The genetic polymorphisms of MBL may be related to inflammatory reaction in the liver of the patients with chronic HCV infection.