人巨细胞病毒感染的检测及临床表现分析

目的 探讨血清人巨细胞病毒（HCMV）-IgM和尿液HCMV-DNA检测对婴、幼儿HCMV感染临床诊断的意义.方法 选择67例确诊为HCMV感染的婴、幼儿,分别采用酶联免疫吸附测定（ELISA）、荧光定量聚合酶链反应（PCR）检测血清HCMV-IgM及尿液HCMV-DNA.结果 HCMV感染的临床表现以肺炎和肝功能异常多见.67例确诊患儿中,血清HCMV-IgM检测的阳性检出率为71.6%,尿液HCMV-DNA检测的阳性检出率为88.1%.二者联合检测,其阳性检出率为95.5%.结论 血清HCMV-IgM和尿液HCMV-DNA联合检查可提高临床诊断的准确性,有助于早期诊断.

Detection of human cytomegalovirus infecion and its clinical manifestations analysis

Objective To explore the significance of serum human cytomegalovirus （HCMV）-IgM and urine HCMV-DNA testing for clinical diagnosis of HCMV infection in infants and young children. Methods Sixty seven infants and young children with definite diagnose of HCMV infection were selected. Enzyme-linked immunosorbent assay（ELISA） and fluorescent quantitative poly- merase chain reaction（PCR） were employed to detect serum HCMV-IgM and urine HCMV-DNA,respectively. Results The major clinical manifestations of HCMV infection were pneumonia and liver function abnormalities. In 67 children with confirmed diagnosis,the positive detection rate of serum HCMV-IgM detection was 71.6% and that of urine HCMV-DNA was 88. 1%. When the two methods were combined,its positive detection rate was 95.5 %. Conclusion The joint detection of serum HCMV-IgM and urine HCMV-DNA can improve the accuracy of clinical diagnosis and will be conducive to early diagnosis.