X射线对人肺腺癌细胞及其耐药细胞株的影响

目的观察人肺腺癌细胞株A549及其耐顺铂细胞株（A549／DDP）对x射线的不同反应，不同放射剂量、照射时间对细胞周期的影响。方法用Vi—Cell细胞活力分析仪绘制A549及A549／DDP生长曲线；分别用4、8、12、16Gv6MVX射线照射A549及A549／DDP，以流式细胞仪检测细胞周期变化。结果A549与A549／DDP存活率分别为83．12％±2．23％，92．00％±2.31％（P〈0．05）。与亲本细胞株相比，A549／DDPGdG，期比例降低（P〈0．01），GgM期比例升高（P〈0．01）。X射线照射后两种细胞出现G2／M期阻滞．照射后24h达到最大阻滞高峰．48h显示最大峰值回落。G2／M期阻滞强度在24h时与放射剂量呈正相关。在4Gy照射后，A549在6h即出现GgM期阻滞，但在照射后12h阻滞解除；而A549／DDP在24h内G2／M期阻滞呈递增趋势。在16Gy照射后，A549及A549／DDP6～12hG2/M期阻滞迅速增加，并持续至24h。结论A549／DDP较其亲本细胞株存活能力更强，对于放射线的敏感性增加。X射线可以改变A549及A549／DDP的细胞周期进程。A549经高放射剂量照射后细胞周期阻滞明显，而A549／DDP无论经过高放射剂量还是低放射剂量照射均表现出明显G2／M期阻滞．

Study of X-ray effect on human lung adenomacarcinoma and its cisplatin -resistance cell lines

Objective To investigate the different influence of X-ray on human lung adenoid cancer cell lines (A549) and its cisplatin-resistant cell line(A549/DDP) and observe the cell cycles at different dosage and irradiation time . Methods The Vi- cell series cell viability analyzer was applied to diagraph the growth curve of them. The A549 and A549/DDP were irradiated by X-ray at different dosage. Cell cycles were checked after the irradiation with flow cytometry. Results The survival rate of A549 and A549/DDP wer...