炒车前子质量标准的研究

目的建立炒车前子Plantaginis Semen Tostum的质量标准。方法 TLC法进行定性鉴别,药典法测定水分、总灰分、酸不溶性灰分含有量及膨胀度,HPLC法测定京尼平苷酸、毛蕊花糖苷含有量。结果 TLC斑点清晰,阴性无干扰。水分、总灰分、酸不溶性灰分平均含有量分别为0.026 6%、4.3%、0.43%,平均膨胀度为4.9。京尼平苷酸、毛蕊花糖苷分别在0.793 75～25.400μg(r=0.999 3)、0.812 5～26.00μg(r=0.999 5)范围内线性关系良好,平均加样回收率分别为99.5%、99.8%,RSD分别为1.2%、2.5%。结论暂定炒车前子水分、总灰分、酸不溶性灰分含有量分别不超过0.032%、5.2%、0.52%,膨胀度及京尼平苷酸、毛蕊花糖含有量分别不少于3.9、0.90%、0.55%。

Quality evaluation for Plantaginis Semen Tostum

AIM To establish the quality standard for Plantaginis Semen Tostum.METHODS TLC was used for qualitative identification, pharmacopeia method was applied to determining water, total ash, acid-insoluble ash contents and turgidity, and HPLC was adopted in the content determination of geniposidic acid and verbascoside.RESULTS The TLC plots were clear without negative interference. The average contents of water, total ash and acid-insoluble ash were 0.026 6%, 4.3% and 0.43%, respectively, along with the average turgidity of 4.9. Geniposidic acid and verbascoside showed good linear relationships within the ranges of 0.793 75-25.400 μg(r=0.999 3) and 0.812 5-26.00 μg(r=0.999 5), whose average recoveries were 99.5% and 99.8% with the RSDs of 1.2% and 2.5%, respectively.CONCLUSION In Plantaginis Semen Tostum, it is temporarily determined that the contents of water, total ash and acid-insoluble ash are not more than 0.032%, 5.2% and 0.52%, turgidity and contents of geniposidic acid, verbascoside are not less than 3.9, 0.90% and 0.55%, respectively.