一测多评法同时测定朴沉化郁丸中9种成分

目的建立一测多评法同时测定朴沉化郁丸(厚朴、陈皮、香附等)中柚皮芸香苷、橙皮苷、川陈皮素、3,5,6,7,8,3′,4′-七甲氧基黄酮、α-香附酮、延胡索乙素、去氢紫堇碱、紫堇碱、四氢小檗碱的含有量。方法该药物70%乙醇提取液的分析采用Waters Xbridge C₁₈色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%冰醋酸,梯度洗脱;体积流量0.9 mL/min;检测波长242、280、300 nm;柱温30℃。以延胡索乙素为内标,计算其他8种成分的相对校正因子,测定其含有量。结果 9种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率为96.99%～99.91%,RSD为0.67%～1.62%。一测多评法所得结果与外标法接近。结论该方法简便稳定,可用于朴沉化郁丸的质量控制。

Simultaneous determination of nine constituents in Pochen Huayu Pills by QAMS

AIM To establish a quantitative analysis of multi-components by single-marker(QAMS) method for the simultaneous content determination of narirutin, hesperidin, nobiletin, 3,5,6,7,8,3′,4′-heptemthoxyflavone, α-cyperone, tetrahydropalmatine, dehydrocorydaline, corydaline and tetrahydroberberine in Pochen Huayu Pills(Magnoliae officinalis Cortex, Citri reticulatae Pericarpium, Cyperi Rhizoma, etc.).METHODS The analysis of 70% ethanol extract of this drug was performed on a 30 ℃ thermostatic Waters Xbridge C18 column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner, and the detection wavelengths were set at 242, 280, 300 nm. Tetrahydropalmatine was used as an internal standard to calculate the relative correction factors of the other eight constituents, after which the content determination was made.RESULTS Nine constituents showed good linear relationships within their own ranges(r>0.999 0), whose average recoveries were 96.99%-99.91% with the RSDs of 0.67%-1.62%. The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This simple and stable method can be used for the quality control of Pochen Huayu Pills.