HMGB2对肺腺癌细胞周期和增殖的影响

目的:探讨高迁移率族蛋白B2(HMGB2)对肺腺癌细胞周期和增殖的影响。方法:从Cancer RNA-Seq Nexus (CRN)数据库分析肺腺癌组织HMGB2表达情况;从OncoLnc数据库分析HMGB2与肺腺癌患者预后的相关性;从肿瘤单细胞数据库(CancerSEA)分析HMGB2与肺腺癌细胞14种功能状态的相关性;利用siRNA技术下调人肺腺癌A549细胞中HMGB2表达,通过real-time PCR和Western blot验证沉默效果,CCK8和EdU实验检测细胞的增殖。结果:HMGB2在肺腺癌中高表达;HMGB2高表达组肺腺癌患者的总生存期明显低于HMGB2低表达患者(log-rank检验P=0.017 3);HMGB2表达与肺腺癌细胞周期和增殖呈正相关;敲减HMGB2表达后A549细胞的活力和增殖能力显著降低(P<0.05)。结论:HMGB2的表达与肺腺癌细胞周期和增殖显著正相关,可以作为潜在的评估肺腺癌患者预后的标志物和治疗靶标。

Effect of HMGB2 on cell cycle and proliferation of lung adenocarcinoma

AIM: To investigate the effect of high-mobility group protein B2(HMGB2) on cell cycle and proliferation of lung adenocarcinoma cells. METHODS: Cancer RNA-Seq Nexus(CRN) was used to analyze HMGB2 expression in lung adenocarcinoma tissues. OncoLnc was used to analyze the correlation between HMGB2 and prognosis of lung adenocarcinoma patients. Cancer Single-cell State Atlas(CancerSEA) was used to analyze the correlation between HMGB2 and 14 kinds of functional states of lung adenocarcinoma. siRNA was used to inhibit HMGB2 expression in human lung adenocarcinoma A549 cells. The silencing effects were verified by real-time PCR and Western blot, and the cell proliferation was detected by CCK8 and EdU assays. RESULTS: HMGB2 was over-expressed in the lung adenocarcinoma tissues. The overall survival of the patients with lung adenocarcinoma in HMGB2 high expression group was significantly lower than that of the patients with low expression of HMGB2(log-rank test P=0.017 3). HMGB2 expression was positively correlated with cell cycle and proliferation of lung adenocarcinoma cells. The viability and proliferation ability of A549 cells after HMGB2 expression knock-down were significantly reduced(P<0.05). CONCLUSION: The expression of HMGB2 is positively correlated with the cell cycle and proliferation of lung adenocarcinoma, and it can be used as a potential marker for evaluating the prognosis and therapeutic target of patients with lung adenocarcinoma.