桑叶多糖超声-微波协同提取工艺优化及其抗氧化活性

目的优化桑叶多糖超声-微波协同提取工艺,并评价其抗氧化活性。方法在单因素试验基础上,以液料比、超声功率、微波功率、协同时间为影响因素,多糖得率为评价指标,响应面法优化提取工艺。考察多糖对DPPH自由基的清除作用。结果最佳条件为液料比25∶1,超声功率139 W,微波功率250 W,协同时间14 min,多糖得率为5.19%。多糖对DPPH自由基具有一定清除能力,IC50为0.5132 mg/mL。结论该方法稳定可靠,可用于超声-微波协同提取抗氧化活性较强的桑叶多糖。

Ultrasonic-microwave synergistic extraction process optimization and anti-oxidant activity for polysaccharides from Mori Folium

AIM To optimize the ultrasonic-microwave synergistic extraction process for polysaccharides from Mori Folium and to evaluate the anti-oxidant activity.METHODS With liquid-solid ratio,ultrasonic power,microwave power and synergistic time as influencing factors,polysaccharides yield as an evaluation index,the extraction process was optimized by response surface method on the basis of single factor test.The scavenging effect of polysaccharides on DPPH free radical was investigated.RESULTS The optimal conditions were determined to be 25∶1 for liquid-solid ratio,139 W for ultrasonic power,250 W for microwave power,14 min for synergistic time,and the polysaccharides yield was 5.19%.The polysaccharides revealed a certain scavenging capacity on DPPH free radical with the IC50 of 0.5132 mg/mL.CONCLUSION This stable and reliable method can be used for the ultrasonic-microwave synergistic extraction of polysaccharides with strong anti-oxidant activity from Mori Folium.