孕康口服液对早发性卵巢功能不全小鼠的影响

目的观察孕康口服液对早发性卵巢功能不全小鼠的影响。方法采用正常动情周期ICR小鼠灌胃给予雷公藤多苷溶液40 mg/kg构建早发性卵巢功能不全模型,大鼠随机分为正常组、模型组及孕康口服液高、中、低剂量组(36、18、9 mL/kg),灌胃给药10周。给药期间定期观察小鼠动情周期;ELISA法检测血清中雌二醇(E₂)、孕激素(P)、促卵泡生成素(FSH)、促黄体生成素(LH)、催乳素(PRL)、抗苗勒管激素(AMH)、抑制素B(INHB)水平;HE染色观察卵巢组织形态;TUNEL染色检测卵巢颗粒细胞凋亡;免疫组化检测Bax、Bcl-2蛋白表达。结果与模型组比较,孕康口服液高剂量组能缩短小鼠动情周期(P<0.05),改善卵巢组织形态学并增加窦状卵泡数量(P<0.05);升高血清E₂水平(P<0.05);减少卵巢颗粒细胞凋亡数量,并能降低Bax表达以及升高Bcl-2表达。结论孕康口服液能改善雷公藤多苷致小鼠早发性卵巢功能不全,其机制可能是通过调节凋亡相关蛋白Bcl-2和Bax的表达,减少卵巢颗粒细胞凋亡。

Effects of Yunkang Oral Liquid on mice with premature ovarian insufficiency

AIM To observe the effects of Yunkang Oral Liquid(YKOL)on mice with premature ovarian in sufficiency(POI).METHODS POI models were induced by intragastric administration of tripterygium wilfordii(40 mg/kg)in ICR mice during normal estrous cycle.The mice were randomly divided into normal control group,model group and high,medium and low dose YKOL groups for 10 weeks oral administration(36,18,9 mL/kg).Periodic observation of mice in estrous cycle was made during the administration;ELISA method was used to detect estradiol(E2),progesterone(P),follicle stimulating hormone(FSH),luteinizing hormone(LH),prolactin(PRL),anti mullerian hormone(AMH)and inhibin B(INHB)levels;HE staining was used to observe the mor phology of ovarian tissue;TUNEL staining was used to detect the apoptosis of ovarian granulosa cells;immunohisto chemistry was used to detect Bax and Bcl 2 protein expression.RESULTS Compared with the model group,the high dose YKOL group shared shortened mice estrous cycle(P<0.05),improved morphology of ovary and in creased number of sinusoidal follicles(P<0.05);increased serum E2 level(P<0.05);reduced number of ovarian granulosa cell apoptosis,and reduced Bax expression and increased Bcl 2 expression.CONCLUSION YKOL can improve the POI induced by tripterygium polyglycoside in mice,and the mechanism may be associated with the apoptosis reduction of ovarian granulosa cells via the Bcl 2 and Bax expression regulation.