| HPLC法同时测定大果飞蛾藤中8种成分 |
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| 引用本文： | 胡静,崔小敏,杨媛媛,任慧,田欢,石会丽,陈志永.HPLC法同时测定大果飞蛾藤中8种成分[J].中成药,2020(3):666-670. |
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| 作者姓名： | 胡静 崔小敏 杨媛媛 任慧 田欢 石会丽 陈志永 |
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| 作者单位： | 陕西省中医药研究院;陕西省中医医院;西安市食品药品检验所 |
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| 基金项目： | 国家自然科学基金面上项目(81973419);国家自然科学基金青年项目(81603264);陕西省创新人才推进计划—青年科技新星项目(2018KJXX-073);西安市科技计划项目[201805103YX11SF37(17),2019115613YX011SF044(13)];陕西省重点研发计划一般项目(2019SF-302,2018SF-302)。 |
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| 摘 要： | 目的建立HPLC法同时测定大果飞蛾藤中新绿原酸、东莨菪苷、绿原酸、隐绿原酸、东莨菪内酯、异绿原酸B、异绿原酸A、异绿原酸C的含有量。方法大果飞蛾藤80%甲醇提取物的分析采用Agilent 5 TC-C18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长345 nm。结果 8种成分在各自范围内线性关系良好(r>0.999 2),平均加样回收率98.22%~101.03%, RSD 0.87%~1.75%。结论该方法准确稳定,重复性好,可用于大果飞蛾藤的质量控制。
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| 关 键 词： | 大果飞蛾藤 化学成分 HPLC |
Simultaneous determination of eight constituents in Porana sinensis by HPLC |
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| HU Jing,CUI Xiao-min,YANG Yuan-yuan,REN Hui,TIAN Huan,SHI Hui-li,CHEN Zhi-yong.Simultaneous determination of eight constituents in Porana sinensis by HPLC[J].Chinese Traditional Patent Medicine,2020(3):666-670. |
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| Authors: | HU Jing CUI Xiao-min YANG Yuan-yuan REN Hui TIAN Huan SHI Hui-li CHEN Zhi-yong |
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| Institution: | (Shaanxi Provincial Academy of Traditional Chinese Medicine,Xi’an 710061,China;Shaanxi Provincial Traditional Chinese Medicine Hospital,Xi’an 710003,China;Xi’an Municipal Institute for Food and Drug Control,Xi’an 710054,China) |
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| Abstract: | AIM To establish an HPLC method for the simultaneous content determination of neochlorogenic acid, scopolin, chlorogenic acid, cryptochlorogenic acid, scopoletin, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C in Porana sinensis Henmsl..METHOD The analysis of 80% methanol extract of P. sinensis was performed on a 25 ℃ thermostatic Agilent 5 TC-C18 column(250 mm×4.6 mm,5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphate acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 345 nm.RESULTS Eight constituents showed good linear relationships within their own ranges(r>0.999 2), whose average recoveries were 98.22%-101.03% with the RSDs of 0.87%-1.75%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of P. sinensis. |
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| Keywords: | Porana sinensis Henmsl chemical constituents HPLC |
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