UPLC-FLD法同时测定不同产地白芷中5种香豆素

目的建立UPLC-FLD法同时测定白芷中水合氧化前胡素、佛手柑内酯、氧化前胡素、欧前胡素、异欧前胡素的含有量。方法白芷甲醇提取物的分析采用Agilent ZORBAX ECLIPSE Plus C₁₈柱(2.1 mm×100 mm,1.8μm);流动相0.1%甲酸水-乙腈;体积流量0.4 mL/min;柱温35℃;荧光检测器激发波长259 nm,发射波长490 nm。结果 5种成分在各自范围内线性关系良好(r=0.999 9),平均加样回收率98.7%～102.5%,RSD 1.02%～4.17%。结论该方法准确稳定,重复性好,可用于白芷的质量控制。

Simultaneous determination of five coumarins in Angelicae Dahuricae Radix from different growing areas by UPLC-FLD

AIM To establish a UPLC-FLD method for the simultaneous determination of oxypeucedanin hydrate,bergapten,oxypeucedanin,imperatorin and isoimperatorin in Angelicae Dahuricae Radix.METHODS The analysis of methanol extract of A.Dahuricae was performed on a 35℃thermostatic Agilent ZORBAX ECLIPSE Plus C18 column(2.1 mm×100 mm,1.8μm),with the mobile phase comprising of 0.1%formic acid water-acetonitrile flowing at 0.4 mL/min in a gradient elution manner,and the excitation wavelength and emission wavelength were set at 259 nm and 490 nm,respectively.RESULTS Five constituents showed good linear relationships within their own ranges(r=0.9999),whose average recoveries were 98.7%-102.5%with the RSDs of 1.02%-4.17%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of A.dahuricae.