四君子颗粒含药血清对棕榈酸诱导的MIN6细胞凋亡的影响

目的探讨四君子颗粒含药血清对棕榈酸诱导的MIN6细胞凋亡的影响。方法SD大鼠灌胃给予四君子颗粒(10.5、5.25 g/kg)7 d,制备四君子颗粒含药血清。四君子颗粒含药血清预处理MIN6细胞24 h后,加入0.4 mmol/L棕榈酸孵育MIN6细胞24 h。CCK-8法检测细胞活力,流式细胞术检测细胞凋亡,Western blot法检测GRP78、ATF4、CHOP、p-PERK蛋白表达。结果四君子颗粒含药血清减轻了棕榈酸诱导的MIN6细胞损伤和凋亡(P<0.05,P<0.01),下调内质网应激相关蛋白GRP78、ATF4、CHOP蛋白表达,降低PERK蛋白磷酸化(P<0.05,P<0.01)。结论四君子颗粒含药血清可有效减轻棕榈酸诱导的MIN6细胞损伤和凋亡,其机制可能与抑制内质网应激相关的信号通路有关。

Effects of Sijunzi Granules medicated serum on MIN6 cell apoptosis induced by palmitic acid

AIM To investigate the effects of Sijunzi Granules(SGs)medicated serum on MIN6 cell apoptosis induced by palmitic acid.METHODS SD rats were given 7-day intragastric SGs administration(10.5,5.25 g/kg)for preparation of SGs medicated serum.MIN6 cells pretreated with 24 h SGs serum were then exposed to 0.4 mmol/L palmitic acid induction for 24 h.The MIN6 cells were subsequently subjected to the detection of cell viability by CCK-8 method,investigation of cell apoptosis by flow cytometry,and detection of GRP78,ATF4,CHOP,and p-PERK protein expression by Western blot.RESULTS SGs medicated serum alleviated injury and apoptosis of palmitic acid-induced MIN6 cells(P<0.05,P<0.01),and down-regulated the expression of endoplasmic reticulum stress-related proteins GRP78,ATF4,and CHOP expression,and reduced PERK protein phosphorylation as well(P<0.05,P<0.01).CONCLUSION SGs medicated serum can effectively reduce MIN6 cells injury and apoptosis induced by palmitic acid-induced,and its mechanism may be related to the inhibition of endoplasmic reticulum stress-related signaling pathways.