车前子对原发性高血压大鼠的降压作用

目的研究车前子对原发性高血压大鼠(SHR)的治疗作用,并探讨其可能的治疗途径。方法SHR分为模型组、阳性药组(2.5 mg/kg)和车前子组(1.3 g生药kg/d),WKY大鼠作为正常组,1 d/次,灌胃8周,每2周测量1次血压。给药8周后,麻醉后腹主动脉取血,检测血清中大鼠Renin、AngⅡ、ACE、ALD、ET-1、eNOS水平;分离胸主动脉,体外检测血管的舒张和收缩功能。结果与正常组比较,模型组中SHR的血压明显升高,血清中Renin、AngⅡ、ACE、ALD和ET-1、eNOS水平均升高,并且血管的收缩功能强于正常组,而舒张功能弱于正常组。与模型组比较,车前子组中SHR的收缩压和血清中Renin、AngⅡ、ACE、ALD的水平均降低,同时紊乱的胸主动脉的收缩和舒张亦得到了改善,升高的ET-1和eNOS的水平均降低。结论车前子对SHR血压有良好的降低作用,其对SHR肾素-血管紧张素-醛固酮系统(RAAS)、ET-1,eNOS的紊乱的改善、对血管舒张和收缩功能障碍的修复作用可能是降低血压的重要途径。

Antihypertensive effect of Plantaginis Semen on spontaneously hypertensive rats

AIM To study the therapeutic effect of Plantaginis Semen(Plantago asiatica L.seed)on spontaneously hypertensive rats(SHR),and to explore its possible therapeutic approaches.METHODS SHR were assigned into model group,positive drug group(2.5 mg/kg)and Plantaginis Semen extract group(1.3 g crude drug/kg).Wistar-Kyoto rats were selected into the normal group.Each group was gavaged once daily for 8 weeks,and had their blood pressure measured every two weeks.After 8 weeks of administration,the rats had their abdominal aorta blood collected after anesthesia,and their serum levels of renin,AngⅡ,ACE,ALD,ET-1 and eNOS measured as well.The isolated thoracic aorta was used for assessing the diastolic and systolic functions of the blood vessels in vitro.RESULTS Compared with the normal group,SHR model group shared increased blood pressure,and higher serum levels of Renin,AngⅡ,ACE,ALD and ET-1,eNOS,stronger vasoconstriction function,and weaker diastolic function.Compared with the model group,Plantaginis Semen extract group presented decreased systolic blood pressure and serum levels of Renin,Ang II,ACE,and ALD,and improved contraction and relaxation of the disordered thoracic aorta,lowered high(previously)levels of ET-1 and eNOS.CONCLUSION Plantaginis Semen has a good effect on reduing hypertension in SHR,and the mechanism may lie in its improvement on the disturbance of SHR RAAS,ET-1,eNOS,and repairing effect on vasodilation and systolic dysfunction.