补益营卫方对衰老皮肤表皮角蛋白18的影响

目的观察补益营卫方对衰老小鼠表皮角蛋白(Keratin,K)18的影响,探讨补益营卫方延缓皮肤衰老的机制。方法传代培养年轻小鼠与自然衰老小鼠表皮细胞,将衰老小鼠表皮细胞分为2组,一组常规培养,一组用含2.5%补益营卫方培养基培养。采用蛋白免疫印迹法(Western blot)及实时荧光定量聚合酶链式反应(Real-time PCR)分别检测细胞K18蛋白、mRNA表达水平。结果与年轻组比较,老年组K18蛋白与mRNA表达水平显著升高(P<0.05);经2.5%药物干预后衰老表皮细胞中K18蛋白与mRNA表达水平较老年组降低(P<0.05)。结论补益营卫方通过抑制衰老皮肤表皮K18蛋白及mRNA表达而延缓皮肤衰老。

Effect of Buyiyingwei Prescription on Epidermal Keratin 18 of Aging Skin

Objective:To observe the effect of Buyiyingwei Prescription on epidermal keratin 18(K18)in aging mice and to explore the mechanism of Buyiyingwei Prescription to delay skin aging.Methods:The epidermal cells of young mice and natural aging mice were subcultured.The senescent mouse epidermal cells were divided into two groups.One group was routinely cultured and the other was cultured with 2.5%tonic Buyiyingwei Prescription medium.Western blot and real-time PCR were used to detect the K18 protein and mRNA expression levels of the cells respectively.Results:Compared with that of the young group,the expression levels of K18 protein and mRNA in the elderly group were significantly increased(P<0.05).The expression levels of K18 protein and mRNA in the senescent epidermal cells were reduced after 2.5%drug intervention compared with that of the elderly group(P<0.05).Conclusion:Buyiyingwei Prescription can delay skin aging by inhibiting the expression of K18 protein and mRNA in aging skin.