天棘膏对小鼠抗疲劳的影响

目的研究天棘膏对小鼠抗疲劳的影响。方法将小鼠随机分为空白组、洋参含片组(洋参含片0.234 g/kg)及天棘膏低、中、高剂量组(天棘膏5.85、11.70、17.55 g/kg),每组12只,1次/d,连续灌胃给药15 d。末次给药1 h后,各组小鼠负重游泳,并记录力竭的时间;无负重游泳90 min后,测定血清中Glu、BLA、TG、TC、BUN水平,肝脏中SOD,GSH-Px,MDA及肌肉Gly水平。结果与空白组比较,天棘膏组低、中、高剂量组小鼠负重游泳时间延长(P<0.05),Glu、Gly水平上调(P<0.05),BLA、BUN的水平降低(P<0.05);高剂量组TG、TC的水平降低(P<0.05);此外,天棘膏中、高剂量组小鼠肝组织中SOD和GSH-Px上调,而MDA的水平下降(P<0.05)。结论天棘膏具有抗疲劳作用,通过调节氧化系统,增强体内抗氧化酶的活性,促进自由基的清除,减少自由基引起的脂质过氧化反应,减少氧化损伤。

Antifatigue effects of Tian-Ji-Gao on mice

AIM To investigate the antifatigue effects of Tian-Ji-Gao on mice.METHODS Mice were randomly divided into blank group, ginseng lozenge group(ginseng lozenge 0.234 g/kg), and low-dose, medium-dose, and high-dose Tian-Ji-Gao groups(Tian-Ji-Gao 5.85, 11.70, 17.55 g/kg) for intragastric administration once daily,for 15 days. One hour after the last dose, the mice in each group swam with heavy loads had their exhaustion time recorded;and after 90-min swimming without heavy load, mice’ serum levels of Glu, BLA, TG, TC and BUN, SOD, GSH-px, MDA in liver and muscle Gly were measured.RESULTS In contrast to mice of the blank group, mice of all Tian-Ji-Gao groups were observed with prolonged weight-bearing swimming time(P<0.05), increased levels of Glu and Gly(P<0.05), and reduced levels of BLA and BUN(P<0.05);and the high-dose group shared reduced TG and TC levels(P<0.05). In addition, mice of both medium-dose and high-dose Tian-Ji-Gao groups displayed up-regulated hepatic SOD and GSH-Px, and decreased MDA levels(P<0.05).CONCLUSION The antifatigue properties of Tian-Ji-Gao may be explained by its regulation upon the oxidation system, enhancement of the antioxidant enzymes activity, promotion of the free radicals elimination, reduction of the free radicals-induced lipid peroxidation, and thereby less oxidative damage.