石榴花化学成分的研究

目的研究石榴Punica granatum L.花的化学成分。方法石榴花60%乙醇提取物采用硅胶、Sephadex LH-20、ODS、半制备液相、重结晶等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到12个化合物,分别鉴定为β-1,4,6-三-O-没食子酰基葡萄糖(1)、butylbrevifolin carboxylate(2)、短叶苏木酚酸甲酯(3)、短叶苏木酚(4)、β-1,6-二-O-没食子酰基葡萄糖(5)、鞣花酸(6)、鹰嘴豆芽素A(7)、retusin-8-methylether(8)、蒲公英赛醇(9)、谷甾醇(10)、β-谷甾醇棕榈酸酯(11)、 crispin A(12)。结论化合物1～3、7～12为首次从石榴属植物中分离得到,化合物1～4、7～12为首次从该植物中分离得到。

Chemical constituents from the flowers of Punica granatum

AIM To study the chemical constituents from the flowers of Punica granatum L..METHODS The 60% ethanol extract from P. granatum was isolated and purified by silica, Sephadex LH-20, ODS, semi-preparative HPLC and recrystallization,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Twelve compounds were isolated and identified as β-1,4,6-tri-O-galloyl-D-glucose(1), butylbrevifolin carboxylate(2), methy brevifolincarboxylate(3), brevifolin(4), 1,6-di-O-galloyl-β-D-glucopyranose(5), ellagic acid(6), biochanin A(7), retusin-8-methylether(8), taraxerol(9), sitosterol(10), β-sitosterol palmitate(11), crispin A(12).CONCLUSION Compounds 1-3,7-12 are isolated from genus Punica for the first time,and compounds 1-4, 7-12 are isolated from this plant for the first time.