软皮热敷散对硬皮病小鼠模型Wnt1和Wnt5a表达的影响

目的观察软皮热敷散对硬皮病模型小鼠皮损中Wnt1、Wnt5a蛋白表达的影响,探讨其治疗硬皮病的作用机制。方法将60只BALB/c小鼠随机分为对照组、模型组、肝素钠组、10%浓度组、20%浓度组、40%浓度组各10只;采用盐酸博莱霉素磷酸缓冲液皮下注射小鼠背部造模,模型组皮下注射生理盐水,1次/d,连续4周,其余40只分别外用肝素钠软膏、10%、20%、40%浓度的软皮热敷散药糊,1次/d,连续4周。观察各组小鼠的皮肤大体形态及组织病理变化;免疫组织化学SABC法检测小鼠皮肤组织中Wnt1与Wnt5a的表达情况。结果模型组真皮厚度较对照组明显增加,差异有统计学意义(P<0.05)。20%、40%浓度组与模型组、肝素钠组及10%浓度组相比真皮厚度明显降低,差异有统计学意义(P<0.05)。20%、40%浓度组与模型组、肝素钠组及10%浓度组相比皮损组织病理情况明显好转。模型组Wnt1、Wnt5a免疫组织化学指数较对照组明显升高,差异有统计学意义(P<0.05)。40%浓度组Wnt1免疫组织化学指数与模型组相比明显降低,差异具有统计学意义(P<0.05)。20%、40%浓度组Wnt5a免疫组织化学指数与模型组比较明显降低,差异有统计学意义(P<0.05)。硬皮病模型小鼠皮损中Wnt1与Wnt5a表达相关系数无统计学意义(P>0.05),两者无相关性。结论 Wnt1、Wnt5a在硬皮病的发病过程中可能具有重要作用,软皮热敷散可能是通过降低皮损中Wnt1、Wnt5a表达从而改善硬皮病模型小鼠的皮肤硬化。

Effects of Soft Leather Hot Casual on the Expression of Wnt1 and Wnt5a in Scleroderma Mice Model

Objective To observe expression of Wnt1 and Wnt5a in the skin lesions of mice model of scleroderma intervened by drug“Soft Leather Hot Casual(SLHC)and to explore the mechanism of its treatment of scleroderma.Methods Sixty BALB/c mices were randomly divided into six groups:control group,model group,heparin group,10%SLHC group,20%SLHC group,40%SLHC group,10 mice in each group.Bleomycin hydrochloride phosphate buffer solution was injected into the back of mice to make model.The model group was hypodermic injected normal saline for 30 days,once a day.The remaining 40 mice were given heparin sodium ointment,10%SLHC,20%SLHC and 40%SLHC paste for 30 days,once a day.The general skin morphology and histopathological changes of mice in each group were observed,and the expressions of Wnt1 and Wnt5a in skin tissue of mice were detected by immunohistochemical SABC method.Results The dermal thickness of the model group was significantly higher than that of the control group(P<0.05).Compared with model group,heparin sodium group and 10%SLHC group,dermal thickness of 20%SLHC group and 40%SLHC group decreased significantly(P<0.05).Compared with model group,heparin sodium group and 10%SLHC group,20%SLHC group and 40%SLHC group showed significant improvement in pathological condition of skin lesions.Immunohistochemical condition:the Wnt1 immunohistochemical index of model group was significantly higher than that of control group.The Wnt1 immunohistochemical index in 20%and 40%SLHC groups were significantly lower than those in model group(P<0.05).Wnt5a immunohistochemical index in 40%SLHC group was significantly lower than that in model group(P<0.05).There was no significant correlation between Wnt1 and Wnt5a expression in scleroderma model mice(P>0.05).Conclusion Wnt1 and Wnt5a may play an important role in the pathogenesis of scleroderma.SLHC may improve the sclerosis of scleroderma model mice by reducing the expression of Wnt1 and Wnt5a in skin lesions.