HPLC法同时测定肠胃散中5种成分

目的建立HPLC法同时测定肠胃散(吴茱萸、陈皮、艾叶等)中5种成分的含有量。方法该药物甲醇提取液的分析采用Agilent C₁₈色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.2%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长215、283 nm。结果橙皮苷、肉桂酸、桂皮醛、吴茱萸碱、吴茱萸次碱分别在45.16～451.6、0.244～2.444、5.07～50.7、2.036～20.36、2.579～25.79μg/mL范围内线性关系良好(r>0.999 0),平均加样回收率98.85%～101.26%,RSD 0.62%～1.19%。结论该方法简便准确,重复性好,可用于肠胃散的质量控制。

Simultaneous determination of five constituents in Changwei Powder by HPLC

AIM To establish an HPLC method for the simultaneous content determination of five constituents in Changwei Powder(Euodiae Fructus,Citri reticulatae Pericarpium,Artemisiae argyi Folium,etc.).METHODS The analysis of methanol extract of this drug was performed on a 25℃thermostatic Agilent C18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of methanol⁃0.2%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 215,283 nm.RESULTS Hesperidin,cinnamic acid,cinnamaldehyde,evodiamine and rutaecarpine showed good linear relationships within the ranges of 45.16-451.6,0.244-2.444,5.07-50.7,2.036-20.36 and 2.579-25.79μg/mL(r>0.9990),whose average recoveries were 98.85%-101.26%,with the RSDs of 0.62%-1.19%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Changwei Powder.