丹参注射剂体内外对糖尿病状态下肾小管AT1表达的影响

目的探究丹参注射剂体内外对糖尿病状态下肾小管AT1表达的影响。方法采用高糖培养基培养NRK-52E,Western blot检测AT1蛋白表达。高糖高脂联合STZ建立糖尿病大鼠模型,大鼠随机分为正常组、模型组、氯沙坦组(20 mg/kg)、丹参注射液组(2 mL/kg),腹腔注射丹参注射剂,1次/d,给药6周。BCA法检测尿蛋白水平、自动生化仪分析检测血尿素氮(BUN)和血清肌酐(Scr)水平,HE染色观察肾脏结构,IHC和Western blot检测AT1蛋白表达。结果丹参注射液可以抑制高糖环境下NRK-52E和糖尿病大鼠肾小管AT1表达的上调(P<0.05,P<0.01),改善糖尿病大鼠24 h尿蛋白排泄(P<0.01),降低血尿素氮(BUN)和血清肌酐(Scr)水平(P<0.01),减轻糖尿病大鼠肾小管病变。结论丹参注射液对糖尿病肾病改善作用可能与抑制AT1表达及改善肾素-血管紧张素系统有关。

Effects of Danshen Injection on AT1 expression in renal tubules under high glucose environment in vivo and in vitro

AIM To investigate the effects of Danshen Injection on AT1 expression in renal tubules under high glucose environment in vivo and in vitro.METHODS We cultured NRK 52E in a high sugar medium,and detected AT1 protein expression by Western blot.Diabetic rat models were established with combinative use of high glucose and high fat diet and STZ.The rats were randomly divided into normal group,model group,losartan group(20 mg/kg),and Danshen Injection groups(2 mL/kg)for intraperitoneal use of Danshen Injection once daily for 6 weeks.The rats were subjected to the detections of urine protein level by BCA method,blood urea nitrogen(BUN)and serum creatinine(Scr)levels by automatic biochemical analyzer,kidney structure by HE.And the AT1 expression was determined by IHC and Western blot.RESULTS Danshen Injection inhibited the up regula tion of AT1 expression in renal tubules of diabetic rats,and so was its impact on NRK 52E in high glucose environ ment(P<0.05,P<0.01).To diabetic rats,Danshen Injection also improved their 24 h urinary protein excretion(P<0.01),reduced their levels of blood urea nitrogen(BUN)and serum creatinine(Scr)(P<0.01),and alle viated their renal tubular lesions.CONCLUSION The improvement of diabetic nephropathy due to Danshen In jection may be associated with its inhibitory effect on AT1 expression and its efficacy in improving renin angiotensin system.