HPLC法同时测定不同产地山茱萸果核中4种成分

目的建立HPLC法同时测定不同产地山茱萸果核中没食子酸、莫诺苷、5-羟甲基糠醛、原儿茶酸的含有量。方法山茱萸果核60%乙醇提取液的分析采用Thermo Hypersil GOLD AQ C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.2%甲酸水溶液,梯度洗脱;体积流量1.0 mL/min;检测波长270 nm;柱温30℃。结果没食子酸、莫诺苷、5-羟甲基糠醛、原儿茶酸分别在7.67~57.51(r=0.999 9)、1.79~13.40(r=0.999 8)、0.78~5.85(r=0.999 8)、5.06~37.98(r=0.999 9)μg/mL范围内线性关系良好,平均加样回收率分别为99.61%、103.74%、98.31%、99.56%,RSD分别为3.00%、2.25%、2.42%、2.09%。结论该方法准确稳定,重复性好,可用于山茱萸果核的质量控制。

Simultaneous determination of four constituents in the fruit core of Cornus officinalis from different growing areas by HPLC

AIM To establish an HPLC method for the simultaneous content determination of gallic acid,morroniside,5-hydroxymethylfurfural,protocatechuic acid in the fruit core of Cornus officinalis Sieb. et Zucc. from different growing areas by HPLC.METHODS The analysis of 60% ethanol extract of the fruit core of C. officinalis was performed on a 30 ℃ thermostatic Thermo Hypersil GOLD AQ C18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.2% aqueous formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 270 nm.RESULTS Gallic acid,morroniside, 5-hydroxymethylfurfural,protocatechuic acid showed good linear relationships within the ranges of 7.67-57.51(r=0.999 9),1.79-13.40(r=0.999 8),0.78-5.85(r=0.999 8),5.06-37.98(r=0.999 9) μg/mL,whose average recoveries were 99.61%,103.74%,98.31%,99.56% with the RSDs of 3.00%,2.25%,2.42%,2.09%,respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of the fruit core of C. officinalis.