HPLC法同时测定白术中4种倍半萜

目的建立HPLC法同时测定白术中白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ、苍术酮的含有量。方法白术甲醇提取液的分析采用Eclipse Plus C18色谱柱(4. 6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱;柱温25℃;体积流量1. 0 m L/min;检测波长220 nm、276 nm。结果白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ、苍术酮分别在0. 005 2~0. 052 mg/m L (R^2=0. 999 6)、0. 010~0. 10 mg/m L (R^2=0. 999 9)、0. 090~2. 9μg/m L (R^2=0. 999 7)、0. 30~1. 8 mg/m L (R^2=0. 999 9)范围内线性关系良好,平均加样回收率99. 13%~100. 34%,RSD 0. 72%~4. 76%。结论该方法准确稳定,重复性好,可用于白术的质量控制。

Simultaneous determination of four sesquiterpenes in Atractylodes macrocephala by HPLC

AIM To establish an HPLC method for the simultaneous content determination of atractylenolide Ⅰ,atractylenolide Ⅱ,atractylenolide Ⅲ and atractylone in Atractylodes macrocephala Koidz.. METHODS The analysis of methanol extract of A. macrocephala was performed on a 25 ℃ thermostatic Eclipse Plus C18 column(4. 6 mm×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelength was set at 220,276 nm. RESULTS Atractylenolide Ⅰ,atractylenolide Ⅱ, atractylenolide Ⅲ and atractylon showed good linear relationships within 0. 005 2-0. 052 mg/m L( R^2= 0. 999 6),0. 010-0. 10 mg/m L( R^2= 0. 999 9),0. 090-2. 9 μg/m L( R^2= 0. 999 7),0. 30-1. 8 mg/m L( R^2= 0. 999 9),respectively,whose average recoveries were 99. 13%-100. 34% with the RSDs of 0. 72%-4. 76%. CONCLUSION This accurate,stable and reproducible method can be used for the quality control of A. macrocephala.