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齐墩果酸对K562细胞系VEGF表达影响的实验研究
引用本文:汤华成,赵蕾,张鹏霞,魏晓东. 齐墩果酸对K562细胞系VEGF表达影响的实验研究[J]. 中国老年学杂志, 2007, 27(24): 2371-2373
作者姓名:汤华成  赵蕾  张鹏霞  魏晓东
作者单位:1. 黑龙江八一农垦大学食品学院,黑龙江,大庆,163319
2. 哈尔滨医科大学大庆校区医学检验系
3. 佳木斯大学基础医学院生物化学教研室
基金项目:黑龙江省自然科学基金资助项目(D200670),黑龙江省卫生厅重点科研项目(2006334),佳木斯大学科研项目(S200603)
摘    要:目的观察齐墩果酸(Oleanolic Acid,OA)对慢性粒细胞白血病系K562细胞中血管内皮生长因子(VEGF)表达的影响。方法采用MTT实验观察OA对体外培养K562细胞增殖抑制作用,用RT-PCR以及Western印迹法研究OA处理后K562细胞中VEGF基因表达的变化。并用ELISA方法检测培养液中VEGF的表达。结果OA能抑制K562细胞生长,呈一定的量效特征;并能抑制K562细胞中VEGF的表达。结论OA通过下调VEGF表达,抑制白血病细胞的增殖。

关 键 词:白血病  齐墩果酸  K562细胞
文章编号:1005-9202(2007)24-2371-03
收稿时间:2007-08-26
修稿时间:2007-11-24

Effect of oleanolic acid on expression of VEGF in human leukaemia cancer cell line K562
TANG Hua-Cheng, ZHAO Lei, ZHANG Peng-Xia,et al.. Effect of oleanolic acid on expression of VEGF in human leukaemia cancer cell line K562[J]. Chinese Journal of Gerontology, 2007, 27(24): 2371-2373
Authors:TANG Hua-Cheng   ZHAO Lei   ZHANG Peng-Xia  et al.
Affiliation:TANG Hua-Cheng, ZHAO Lei, ZHANG Peng-Xia, et al.
Abstract:Objective To observe the effect of oleanolic acid on expression of vascular endothelial growth factor(VEGF) in human leukaemia cancer cell line K562.Methods The inhibitory effect of oleanolic acid on viability of K562 cells was assessed by MTT assay.The expression of VEGF mRNA and protein were determined by RT-PCR and Western blot,respectively.The expression of VEGF in culture medium was detected by ELISA.Results Oleanolic acid inhibited growth of K562 cells in a dose-dependent manner and down-regulated expression of VEGF.Conclusions The anti-tumor mechanism of oleanolic acid is related with inhibition of VEGF expression,which causes down-regulation of VEGF,therefore decreases tumor growth.
Keywords:VEGF
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