乌司他丁对窒息大鼠心肺复苏后氧自由基和心功能变化的干预研究

目的研究心脏骤停大鼠心肺复苏后血清和心肌的氧自由基表达与左心功能的变化，并探讨鸟司他丁的干预作用。方法随机将SD大鼠分为三组：假手术组（不进行窒息及心肺复苏，仅进行麻醉和气管切开插管、血管穿刺，S组）；对照组（静脉注射生理盐水4mL．kg-1，C组）;考察组（鸟司他丁5万U·kg-1，给药容积为4mL·kg-1，UTI组）。分别测定：（1）每只大鼠术前、心肺复苏后6h血清超氧化物歧化酶（SOD）、丙二醛（MDA）、左心室内压最大上升厦下降速率（士LVdp／dt—max）；（2）各组心肺复苏术后6h心肌组织SOD、MDA的表达。结果（1）C组、UTI组复苏术后6h血清SOD、±LVdp／dtmax较术前及S组降低（P-（0．05）；C组、UTI组复苏后6h血清MDA高于术前及S组（P〈0．05）。（2）心肺复苏后6hS组、UTI组、C组间血清SoD、血清MDA、土LVdp／dt—max、心肌SOD、心肌MDA组间比较差异有统计学意义（P〈0．05）。（3）心肺复苏后6h士LVdp／dt．max与心肌SOD水平呈正相关、与心肌MDA水平呈负相关，差异均有统计学意义（P〈0．05）。结论鸟司他丁可抑制氧化应激损伤，改善心搏骤停-心肺复苏实验大鼠心功能不全。

Study on the effects of ulinastatin on the expression of oxygen radicals and heart function protection after cardiopulmonary resuscitation in rats.

Objective To investigate the effects of ulinastatin on the expression of oxygen radicals and heart function protection after cardiopulmonary resuscitation in rats with anoxia- induced cardiac arrest. Methods 50 Male Sprague Dawley （SD） rats were randomly divided into three experimental groups, which were sham operation group （Group S,n = 8,only anesthesia, tracheotomy tube and vas- cular puncture）, control group （Group C, n = 6, anoxia- induced cardiac arrest - cardiopulmonary resus- citation and physiological saline 4 mL/kg was injected via vein）, and ulinastatin treatment group （Group UTI, n = 6, anoxia- induced cardiac arrest- cardiopulmonary resuscitation and Ulinastatin 50 000 U/kg was injected via vein）. The concentrations of serum superoxide dismutases （SOD）,ser- um malondialdehyde （MDA） and the maximum rising and failing rates of left ventricular deep pressure （ ± LVdp / dt max） were detected at setting time before and 6 h after cardiopulmonary resuscitation in rats. The expression of myocardium SOD and MDA of 6 h after cardiopulmonary resuscitation in rats was observed. Results Compared with sham operation group and before cardiopulmonary resuscitation, the concentrations of SOD and ±LVdp/dtmax decreased （P〈0.05） while the concentrations of MDA increased （P〈0.05） at 6 h after cardiopulmonary resuscitation in Group C,UTI. In addition,different degrees of myocardial damage are discovered under the microscope. The concentrations of SOD, MDA, ± LVdp / dt max and the expression of myocardium SOD, MDA of 6 h after cardiopulmonary resusci- tation were significantly （P〈0.05） while group comparison. There was a positive correlation of the ± LVdp/dtmax levels with the expression of myocardium SOD while the relation of the ± LVdp/dt- max levels with the expression of myocardium MDA was negative correlation. Conclusion Ulinastatin can improve cardiac dysfunction by mitigate oxidative stress injury in rats with anoxia- induced cardiac arrest - cardiopulmonary resuscitation.