肺动脉高压大鼠骨髓内皮祖细胞功能的研究

目的检测实验性肺动脉高压大鼠骨髓内皮祖细胞（EPC）的功能,探讨肺动脉高压发病机制。方法利用野百合碱诱导大鼠发生肺动脉高压,分离骨髓单个核细胞进行体外诱导培养以获得EPC集落,并对其骨髓内皮祖细胞的集落形成能力、增殖、黏附、迁移能力进行检测。结果骨髓单个核细胞在体外培养下能够获得EPC集落,与对照组比较,肺动脉高压实验组诱导生成的EPC数量少（P〈0.05）,CD34和FLK-1阴性比例下降（分别为19.33%±3.27%vs 31.17%±4.40%和33.67%±3.50%vs 44.50%±3.78%,P均〈0.01）,细胞增殖能力下降（0.43±0.08 vs 0.64±0.07,P〈0.01）,贴壁细胞减少（6.835个±1.605个vs 10.175个±1.945个,P〈0.01）,细胞迁移能力下降（7.83个±1.94个vs 11.83个±2.48个,P〈0.05）。结论肺动脉高压的发生与骨髓内皮祖细胞功能的异常存在明显的相关性。

Function of bone marrow endothelial progenitor cell in rat model with pulmonary hypertension

Objective To assess the function of bone marrow derived endothelial progenitor cell（EPC） with pulmonary hypertension in experimental rat model,and investigate the pathogenesis of pulmonary hypertension.Methods The rats were injected with crotaiine to induce pulmonary hypertension,then separate bone marrow mononuclear cell and cultured in vitro to obtain EPC colony.The ability of EPC colony forming,proliferate,adhesion and migration were assessed.Results EPC colony could be attained from bone marrow mononuclear cell in vitro.Compared with control group,the amount of EPC induced in experimental group was less（P0.05）,the negative proportion of CD34 and FLK-1 was lowered（19.33%±3.27%vs 31.14%±4.40%and 33.67%±3.50%vs 44.50%±3.78%respectively,P0.01 ）,the proliferate ability decreased（0.43±0.08 vs 0.64±0.07,P0.01 ）,adherent cells decreased（6.835±1.605 vs 10.175±1.945,P0.01 ）,migration ability weakened（7.83±1.94 vs 11.83±2.48,P0.05）.Conclusion The incidence of pulmonary hypertension was associated with abnormal function of bone marrow endothelial progenitor evidently.