利用基因转染技术长期培养人甲状腺细胞

本工作采用Ｃａ３（ＰＯ４）２ＤＮＡ共沉淀法，将含有编码ＳＶ４０病毒早期基因的ｐＳＧ５质粒转染原代培养的人甲状腺上皮细胞。在重复进行的１２次转染中，有１次细胞生存达２００ｄ，传了２６代。检测证实，转染后的细胞仍能保持少量Ｔ４的分泌和Ｔｇ的合成；细胞经长期培养后形态无异常，Ｇ带染色正常，对裸鼠无致瘤性。甲状腺滤泡上皮细胞长期培养模型的建立，为进一步研究细胞免疫在自身免疫性甲状腺炎中的作用打下了基础。

Long term culture of human thyroid epithelial cells with gene transfection

By using calcium phosphate co precipitation techniques, primary cultured of thyroid epithelial cells were transfected by pSG5, a plasmid containing SV40 early region genome. In one of the twelve transfections, thyroid epithelial cell culture survived for 200 days, for 26 passages. T 4 and thyrogloblin(Tg) determinations showed that the transfected cells maintained the secretion of T 4 and Tg constantly, and the secretion level was similar to original culture. These cells had epithelial morphology, normal karyotypic and no tumorigenicity to nude mice. This human epithelial cells long term culture model might be useful for the study of autoimmune thyroiditis pathogenesis.