重组异种骨二次免疫的实验研究

目的：研究重组异种骨（ＲＢＸ）重复植入动物体内引起的二次免疫应答及其对成骨活性的影响。方法：将ＲＢＸ间隔不同时间重复植入雄性Ｂａｌｂ／ｃ小鼠股部肌肉内，通过ＥＬＩＳＡ测定体液免疫水平，检测局部组织的碱性磷酸酶（ＡＬＰ）活性反映成骨情况。结果：ＲＢＸ第１次植入后，血清中可检测到特异性抗体；局部组织中的ＡＬＰ活性略低于正常小鼠骨骼。间隔３周或６周再次植入ＲＢＸ，血清中特异性抗体水平明显增高（Ｐ＜０．０５）；ＡＬＰ活性降低（Ｐ＜０．０１）。结论：重复植入ＲＢＸ可诱发机体产生免疫应答；ＲＢＸ诱导成骨活性受到抑制，成骨作用延迟。

Studies on secondary immune response of reconstituted bone xenograft

Aim: The immune response to reconstituted bone xenograft (RBX) and its effect on bone induction in animal models following second set grafting were investigated. Methods: Enzyme linked immunosorbent assay were performed in order to determine the levels of humoral immunity, and the local ALP were characterized to show the degree of bone induction and formation. Results: RBX could stimulate the mice to produce specific antibodies to RBX, and there more rapid development and higher levels of antibodies after second set graft of RBX compared with the levels obtained after first set graft. Conclusion: Repeated grafting of RBX to the body seems to inhibit and delay its osteoinductive activities.