降钙素原和白介素-6在感染性疾病病原体诊断中的联合检测价值

目的 探讨降钙素原（PCT）与白介素-6（IL-6）两种血清学标志物在感染性疾病,病原体种类检测中价值.方法 采用上转发光免疫分析仪及配套试剂检测79例细菌感染性疾病患者、59例病毒感染性疾病患者和68例健康体检者血清样本中PCT和IL-6含量,应用受试者工作特征（ROC）曲线进行分析.结果 细菌感染组PCT和IL-6含量高于病毒感染组及正常对照组,差异具有统计学意义（P＜0.05）,病毒感染组PCT和IL-6含量与正常对照组比较差异不具统计学意义（P＞0.05）;应用ROC曲线分析PCT和IL-6鉴别诊断细菌感染的效能,曲线下面积分别为0.854和0.783,显示PCT效能优于IL-6;对三组的PCT与IL-6水平进行了相关性分析,结果显示,PCT与IL-6在检测细菌感染中成线性相关（r=0.424,P＜0.001）;对两项指标进行单项检测时,PCT的灵敏度和特异性分别是82.3％和79.5％,IL-6的灵敏度和特异性分别是69.6％和68.5％;二者联合检测时灵敏度和特异性是88.6％和67.0％.结论 在病原微生物的感染诊断中,PCT是一个优秀指标,若能与IL-6联合检测,更有利于细菌感染性疾病与病毒感染性疾病的鉴别诊断.

Clinical significance of joint detection of procalcitonin and interleukin-6 in the pathogen diagnosis of infectious disease

Objective To study the changes of serum procalcitonin （PCT） and interleukin-6 （IL-6） in the clinical value in the pathogen diagnosis of infectious disease.Methods Using the Up-converting Phosphor Technology based Lateral Flow assay （UPT） to detect 79 cases of bacterial infections,59 cases of viral infectious diseases and 68 cases of healthy normal human serum PCT,IL-6 content,and using receiver operating characteristic（ROC） curve to analyse.Results Bacterial infection group PCT and IL-6 were significantly higher than that of virus group and normal control group,there was significant difference （P 〈0.05）,PCT and IL-6 in virus group and normal control group virus infection were not significantly different （P 〉 0.05） ; application analysis of ROC curves of PCT and IL-6 in diagnosis and differential diagnosis of bacterial infection efficiency,the area under the curve were 0.854 and 0.783,PCT was more effective than IL-6 ; PCT and IL-6 in the detection of bacterial infection,a linear correlation （r =0.424 P 〈 0.001） ; single PCT detection,the sensitivity and specificity were 82.3% and 79.5%,the sensitivity and specificity of IL-6 were 69.6% and 68.5%,the combination of the two detection sensitivity and specificity were 91.1% and 67%.Conclusion In the diagnosis of pathogen infection,PCT is a good index,if combined with IL-6 detection,differential diagnosis is more conducive to bacteria,virus infection disease.