| 噬菌体肽库在宫颈癌血清肿瘤标志物筛选中的应用 |
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| 引用本文： | 刘美红,贺海平. 噬菌体肽库在宫颈癌血清肿瘤标志物筛选中的应用[J]. 现代肿瘤医学, 2015, 0(7): 908-910 |
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| 作者姓名： | 刘美红 贺海平 |
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| 作者单位： | 广西医科大学附属肿瘤医院肿瘤内科;广西医科大学附属肿瘤医院信息中心 |
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| 基金项目： | 广西科技厅基金项目(编号:0991232) |
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| 摘 要： | 目的:用噬菌体随机12肽库对宫颈癌患者血清进行差异性筛选,筛选出能与宫颈癌患者血清特异性结合的肿瘤标志物。方法:应用噬菌体随机12肽库对宫颈癌患者和正常人血清进行三轮差异性筛选,用ELISA法检测噬菌体克隆对宫颈癌患者血清结合的特异性。结果:经过三轮筛选,噬菌体富集率逐轮提高,提高近100倍。用ELISA法对50例宫颈癌患者血清和50例健康者血清检测验证,其中获得一株与宫颈癌患者血清特异结合较好的噬菌体,对宫颈癌的早期诊断具有潜在价值。结论:筛选出能与早期宫颈癌患者血清高亲和力特异结合的短肽,为研究宫颈癌肿瘤标志物及宫颈癌的早期诊断奠定了基础。
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| 关 键 词： | 宫颈癌 噬菌体肽库 肿瘤标志物 |
Application of phage display peptide library in screening of the tumor markers in the ser-um of cervical cancer |
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| Liu Meihong;He Haiping. Application of phage display peptide library in screening of the tumor markers in the ser-um of cervical cancer[J]. Journal of Modern Oncology, 2015, 0(7): 908-910 |
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| Authors: | Liu Meihong He Haiping |
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| Affiliation: | Liu Meihong;He Haiping;Department of Medical Oncology,Tumor Hospital Affiliated to Guangxi Medical University;Information Center,Guangxi Tumor Hospital; |
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