祛瘀生肌法对SD大鼠创面模型新生肉芽组织中转化生长因子-β的动态影响

目的：探讨祛瘀生肌法对SD大鼠愈创模型肉芽组织中转化生长因子-β（TGF-β）水平的动态影响。方法：采用生肌化瘀方干预SD大鼠背部全层皮肤缺损模型，分别于不同干预时段（3d、7d、11d）取材，EnVision二步法免疫组化测定，并图像扫描定量分析TGF-β1、TGF-β3、水平变化。结果：第3、第7天生肌化瘀方组TGF-β1水平均高于生理盐水组（P〈0．05），第11天生肌化瘀方组TGF-β1水平低于生理盐水组（P〈0．05）；第3天生肌化瘀方组TGF—β3水平低于生理盐水组，而第7、第11天TGF-β3水平高于生理盐水组（P〈0．05）。结论：生肌化瘀方有调节创面TGF—β1、TGF—β3分泌的作用，以益于加速创面愈合、减少瘢痕形成。

Dynamic Influence of Stasis-Removing and Muscle-Producing Therapy on Expression of TGF-β in Granulation Tissue of Rat Wounds

Objective： To investigate the dynamic influence of stasis-removing and muscle-producing therapy on expression of transforming growth factor-β （TGF-β） in granulation tissue of rat wounds. Methods： The model of SD rats with full-thickness skin defect on the back was treated with the stasis-removing and muscle-producing therapy and the specimens were collected on the third, seventh and eleventh days after wounding. The expression of TGF-β1 and TGF-β3 were determined with EnVision immunohistoehemieal technique and image analysis. Results： On the third and senenth days, the expression of TGF-β1 of ＂ShengJi Huayu Recipe＂ （SJHY） Group was significantly higher than that of control group（P〈0.05）： and on the eleventh day, the expression of the TGF-β1 of SJHY group was significantly lower than that of control group（P〈 0.05）; on the third day, the expression of TGF-β3 of SJHY Group was significantly lower than that of control group（P〈 0.05）;on the day 7 and day ll,the expression of the TGF-β3 of SJHY group was significantly higher than that of control group （P 〈 0.05）. Conclusion： Stasis-removing and muscle-producing therapy can regulate the secretion of TGF-β1 and TGF-β3 in wound, thus accelerating the wound healing and reducing the scarring.