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Development of Peptidyl Lysine Dendrons: 1,3‐Dipolar Cycloaddition for Peptide Coupling and Antibody Recognition |
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| Authors: | Christine Hüttl Cornelia Hettrich Melanie Riedel Petra Henklein Harshadrai Rawel Frank F. Bier |
| Affiliation: | 1. Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (Fraunhofer IZI‐BB), Potsdam, Germany;2. Institute for Biochemistry und Biology, University of Potsdam, Potsdam, Germany;3. Institute of Biochemistry, Charité – Universit?tsmedizin Berlin, Berlin, Germany;4. Institute of Nutritional Sciences, University of Potsdam, Nuthetal, Germany |
| Abstract: | A straightforward synthesis strategy to multimerize a peptide mimotopes for antibody B13‐DE1 recognition is described based on lysine dendrons as multivalent scaffolds. Lysine dendrons that possess N‐terminal alkyne residues at the periphery were quantitative functionalized with azido peptides using click chemistry. The solid‐phase peptide synthesis (SPPS) allows preparing the peptide dendron in high purity and establishing the possibility of automation. The presented peptide dendron is a promising candidate as multivalent ligand and was used for antibody B13‐DE1 recognition. The binding affinity increases with higher dendron generation without loss of specificity. The analysis of biospecific interaction between the synthesized peptide dendron and the antibody was done via surface plasmon resonance (SPR) technique. The presented results show a promising tool for investigations of antigen–antibody reactions. |
| Keywords: | click chemistry lysine dendron peptide mimotopes solid‐phase peptide synthesis surface plasmon resonance |