Fibroblast growth stimulation by extracts and compounds of Onosma argentatum roots |
| |
Authors: | Ozgen U Ikbal M Hacimuftuoglu A Houghton P J Gocer F Dogan H Coskun M |
| |
Institution: | Atatürk University, Faculty of Pharmacy, Department of Pharmacognosy, 25240 Erzurum, Turkey. uozgen@atauni.edu.tr |
| |
Abstract: | The roots of Onosma argentatum are used traditionally in Turkey for wound healing and burns. The n-hexane-dichloromethane extract of the roots, and four shikonin derivatives (deoxyshikonin, acetyl shikonin, 3-hydroxy-isovaleryl shikonin and 5,8-O-dimethyl acetyl shikonin) isolated from the n-hexane-dichloromethane extract were investigated for their ability to stimulate the growth of human amnion fibroblasts. A range of concentrations was studied and the extract found to stimulate the growth of human amnion fibroblasts in vitro at 0.1 microg/mL whilst 5,8-O-dimethyl acetyl shikonin had the same effect at 0.05-5 microg/mL, although cytotoxicity was observed at 50 microg/mL for all samples. The extract and all the other isolated compounds showed cytotoxicity at 10 microg/mL with the extract and 3-hydroxy-isovaleryl shikonin showing cytotoxicity at 5 microg/mL. It is suggested that any wound healing effect of the roots of Onosma argentatum might be partly due to an additive effect of the shikonin derivatives present. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|