酪氨酸转氨酶法制备L-高苯丙氨酸

目的为抗高血压新药的共同中间体L-高苯丙氨酸的制备奠定基础。方法构建工程菌E.coli BL21(DE3)pET 30a-tyrB,乳糖诱导表达酪氨酸转氨酶;利用酪氨酸转氨酶对底物2-氧-4-苯基丁酸进行转化;利用HPLC、MS、1H-NMR进行产物检测与结构鉴定。结果构建了工程菌E.coliBL21(DE3)pET 30a-tyrB,乳糖诱导酪氨酸转氨酶表达,制备得到L-高苯丙氨酸。结论利用转氨酶催化法可以高效制备L-高苯丙氨酸。

Synthesis of L-homophenylalanine by tyrosine aminotransferase method

Objective To lay the foundation for efficient preparation of L-homophenylalanine,which is a common intermediate for antihypertensive drugs.Methods The engineering bacteria E.coli BL21(DE3)pET 30a-tyrB was established first,then the tyrosine aminotransferase was induced by lactose and the transformation of 2-oxo-4-phenyl butyric acid to L-homophenylalanine was accomplished by the enzyme mentioned above.HPLC,MS,1H-NMR were used to indentify the products.Results The engineering bacteria were established and L-homophenylalanine was synthesized by catalysis of tyrosine aminotransferase.Conclusions The aminotransferase is a potential and effective tool for the synthesis of L-homophenyalanine.