Cytokeratin 5/6 in normal human breast: lack of evidence for a stem cell phenotype |
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Authors: | Clarke Catherine L Sandle Jennifer Parry Suzanne C Reis-Filho Jorge S O'Hare Michael J Lakhani Sunil R |
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Affiliation: | Breakthrough Toby Robins Breast Cancer Research Centre, Institute of Cancer Research, London, UK. |
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Abstract: | In recent studies, B?cker and colleagues described a population of cells in paraffin wax sections of normal human breast that express cytokeratins (CK) 5/6 without expression of CK8/18 or smooth muscle actin (SMA). They proposed that these represent stem cells that give rise to differentiated luminal and myoepithelial cells. The data have been used to generate a model for breast cancer progression and classification with associated implications for management of pre-invasive disease. In this study, the expression of CK5/6, CK8/18, and SMA was investigated using multiple immunofluorescence on matched pairs of paraffin wax-embedded and frozen breast specimens. The staining patterns reported previously in antigen-retrieved paraffin wax-embedded sections were confirmed but no CK5/6-only cells were found in frozen sections of normal breast. There were cells with low levels of CK8/18 expression in frozen sections that may correspond to the CK8/18 'negative' cells seen in paraffin wax sections. This study brings into question the previously described profile of breast 'stem cells' based on CK5/6 staining and hence the breast cancer progression model and classification based on this phenotype. |
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Keywords: | breast stem cells cytokeratin 5/6 antigen retrieval immunofluorescence |
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