葡萄籽原花青素对兔心肌再灌注损伤诱发的细胞凋亡保护作用

目的：探讨葡萄籽原花青素（grape seed proanthocyanidin extract，GSPE）对兔实验性心肌再灌注损伤（myocardial reperfusion injury，MRI）诱发的心肌细胞凋亡的保护作用。方法：建立兔在体心肌再灌注损伤模型，24只兔随机平分为4组：假手术对照组（A）、缺血再灌注模型组（B）分别用生理盐水灌胃，低剂量GSPE治疗组（C）用GSPE（100?mg/kg）灌胃，高剂量GSPE治疗组（D）用GSPE（200?mg/kg）灌胃，共喂养21?d。各组测定再灌注前后不同时间血清心肌肌酸激酶同工酶MB（CK MB）、超氧化物歧化酶（SOD）、丙二醛（MDA）含量，对照观察各组兔心肌超微结构变化及心肌细胞凋亡情况。结果：与B组相比， C、D组的血清CK MB和MDA显著降低（P＜0.05，P＜0.01)，血清SOD明显升高（P＜0.01)；病理分析显示，C、D组兔心肌细胞损伤明显减轻，心肌细胞凋亡的数目明显减少，D组凋亡指数（AI）明显降低（P＜0.01)。结论：GSPE在减轻兔MRI的同时，对其引起的细胞凋亡有明显的保护作用，可能是其抗MRI的机制之一。

Anti-apoptosis effect of grape seed proanthocyanidin extract on experimental myocardial reperfusion injury in rabbits

Objective: To study the effect of grape seed proanthocyanidin extract(GSPE) on the apoptosis of myocardial cells induced by myocardial reperfusion injury(MRI) in rabbits.Methods: Twenty-four male New Zealand rabbits were divided into four groups at random: control,model,low dose and high dose groups.After 21 days of treatment,serum CK-MB,SOD and MDA of the rabbits of each group were detected at different times before and after reperfusion,the ultra-structure of myocardium and the apoptosis of myocardial cells were observed.Results: The CK-MB and MDA decreased,but SOD increased in GSPE group compared with the model group(P<0.01,P<0.05).Pathological analysis manifested MRI revived markedly in GSPE group.The amount of apoptotic cells and the apoptosis index(AI) reduced significantly in GSPE group(P<0.01).Conclusion: GSPE has a significantly preventive effect on myocardial cell apoptosis induced by MRI,except for its potent antioxidant activity,which may be one of the mechanisms of its anti-MRI effect.