| RP-HPLC法测定石楠叶中熊果酸和齐墩果酸的含量 |
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| 引用本文： | 罗晓清,祝祎玮,钱苏生. RP-HPLC法测定石楠叶中熊果酸和齐墩果酸的含量[J]. 现代中药研究与实践, 2003, 17(5): 14-15 |
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| 作者姓名： | 罗晓清 祝祎玮 钱苏生 |
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| 作者单位： | 江苏苏州卫生学校,江苏,苏州,215002 |
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| 摘 要： | 目的 建立石楠叶中熊果酸和齐墩果酸的含量测定方法。方法 采用 RP- HPL C法测定 ,色谱柱为Zorbax ODS柱 ,用甲醇 - 1%醋酸水溶液 (88∶ 12 )为流动相 ,检测波长为 2 15 nm。结果 熊果酸和齐墩果酸的平均回收率分别为 98.5 % ,97.6 % (n=3) ,RSD分别为 1.6 2 ,1.89 (n=3)。结论 本法准确、灵敏、快速 ,可作为石楠叶药材的质量检测方法之一。
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| 关 键 词： | RP-HPLC法 石楠叶 熊果酸 齐墩果酸 |
| 文章编号： | 1004-2199(2003)05-0014-02 |
| 修稿时间： | 2003-08-19 |
Determination of Ursolic Acid and Oleanolic Acid in Leaves of Photinia serrulata by RP-HPLC |
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| LUO Xiao-qing,ZHU Yi-wei,QIAN Su-sheng. Determination of Ursolic Acid and Oleanolic Acid in Leaves of Photinia serrulata by RP-HPLC[J]. Research and Practice on Chinese Medicines, 2003, 17(5): 14-15 |
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| Authors: | LUO Xiao-qing ZHU Yi-wei QIAN Su-sheng |
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| Abstract: | Objective To establish a method for the determination of ursolic acid and oleanolic acid in leaves of Photinia serrulata.MethodThe determination was carried out by RP-HPLC on Zorbax ODS column (4.6 mm×250 mm,5 μm) with methol-1 %HAc solution (88∶12) as the mobile phase, and the detective wavelength was 215 nm.Results The average recovery of ursolic acid and oleanolic acid was 98.5 and 97.6% (n=3) with RSD of 1.62% and 1.89% (n=3),respectively.Conclusion This method is rapid , simple , accurate and good for the determination of ursolic acid and oleanolic acid in leaves of Photinia serrulata. |
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| Keywords: | RP-HPLC Photinia serrulata Lindl ursolic acid oleanolic acid |
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