洛伐他汀对大鼠角膜新生血管VEGF和TGF-β1表达的影响

目的:探讨洛伐他汀(lovastatin)对大鼠角膜新生血管(cor-neal neovascularization,CNV)血管内皮生长因子(vascular endothelial growth factor,VEGF)和转化生长因子-β1(trans-forming growth factor-beta1,TGF-β1)表达的影响。方法:建立碱烧伤诱导的大鼠角膜新生血管的动物模型,治疗组和对照组分别给予10-5mol/L洛伐他汀溶液和生理盐水点眼,采用裂隙灯观察新生血管的生长情况,用免疫组织化学方法检测VEGF及TGF-β1的表达,并在基因水平下采用RT-PCR方法检测大鼠角膜VEGF mRNA的表达水平。结果:各时间点角膜新生血管的面积,治疗组均低于对照组,差异有显著性(P<0.05),免疫组化染色显示,治疗组VEGF及TGF-β1的表达量较对照组明显减少,在造模后4d角膜组织中VEGF mRNA的相对表达量,治疗组低于对照组(0.422±0.083vs0.786±0.126,P<0.05)。结论:洛伐他汀通过下调VEGF及TGF-β1的表达,抑制血管内皮细胞增殖,最终可有效抑制碱烧伤诱导的大鼠角膜新生血管形成。

Effect of Iovastatin on the expression of VEGF and TGF-β1 of corneal neovascularization of rats

AIM: To investigate the effect of lovastatin on the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-beta1 (TGF-β1) in corneal neovascularization (CNV) of rats.·METHODS: Corneal neovascularization model was established by alkaline burning. CNV model rats were randomly divided into two groups: the experimental group received 10-5mol/L lovastatin drops, and the control group was treated with physiologic saline. The growth of neovascularization was observed under slit lamp microscopy. The expression of VEGF and TGF-β1 in CNV of each rat were detected by immunohistochemical staining. And the VEGF mRNA expression in rat’s cornea was measured by RT-PCR.·RESULTS: Compared with the control group, the growth of CNV in experimental group at various time points was reduced significantly (P<0.05). Immunohis-tochemistry showed that the expression of VEGF and TGF-β1 in experimental group was lower than that in control group. At 4 days of injury, the expression of VEGF mRNA in the cornea of experimental rats was decreased compared to with that of control rats (0.422±0.083 vs 0.786±0.126, P<0.05).·CONCLUSION: Lovastatin can effectively inhibit the growth of CNV induced by alkaline burning through reducing the expression of VEGF and TGF-β1 in cornea.