Ganglioside potentiation of NGF-independent conditioned medium enhancement of neuritic outgrowth from spinal cord and ciliary ganglia explants |
| |
| Authors: | P E Spoerri F J Roisen |
| |
| Institution: | 1. Department of Hematology and Clinical Immunology, Yokohama City University Graduate School of Medicine, Yokohama, Japan;2. Department of Hematology, Kanagawa Cancer Center, Yokohama, Japan;3. Department of Hematology, Yokohama City University Medical Center, Yokohama, Japan;4. Division of Metabolism and Biosystemic Science, Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Asahikawa, Japan;5. Japanese Red Cross Hokkaido Blood Center, Sapporo, Japan;6. Department of Biostatics, Yokohama City University Graduate School of Medicine, Yokohama, Japan;1. Graduate Program for Collective Health, Faculty of Health Sciences, University of Brasilia (UnB), Brasilia, Brazil;2. Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil;3. Departament of Hemotherapy and Cell Therapy, Israelita Albert Einstein Hospital, São Paulo, Brazil;4. Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;5. Medical School, State University of Rio de Janeiro, Rio de Janeiro, Brazil;6. Collective Health School, Faculty of Ceilandia, University of Brasilia, Brasília, Brazil;1. Department of Hematology/Oncology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan;2. Department of Hematology and Oncology, Anjo Kosei Hospital, Anjo, Japan;3. Division of Hematology, Jichi Medical University Saitama Medical Center, Saitama, Japan;4. Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, Japan;5. Hematopoietic Stem Cell Transplantation Division, National Cancer Hospital, Tokyo, Japan;6. Department of Hematology, Kanagawa Cancer Center, Yokohama, Japan;7. Department of Hematology, Japanese Red Cross Aichi Medical Center Nagoya Daiichi Hospital, Nagoya, Japan;8. Department of Hematology, Sapporo Hokuyu Hospital, Sapporo, Japan;9. Department of Hematology, National Hospital Organisation Kumamoto Medical Center, Kumamoto, Japan;10. Department of Hematology, Toranomon Hospital, Tokyo, Japan;11. Department of Hematology, Hiroshima Red Cross Hospital & Atomic-Bomb Survivors Hospital, Hiroshima, Japan;12. Department of Hematology, Kobe City Medical Center General Hospital, Kobe, Japan;13. Department of Hematology, Hamanomachi Hospital, Fukuoka, Japan;14. Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan;15. Japanese Data Center for Hematopoietic Cell Transplantation, Nagakute, Japan;16. Department of Registry Science for Transplant and Cellular Therapy, Aichi Medical University School of Medicine, Nagakute, Japan;17. Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan |
| |
| Abstract: | Culture medium conditioned (CM) by embryonic chick skeletal muscle or RN22 Schwannoma cells enhanced dramatically the neuritic development of chick embryonic spinal cord slices explanted onto a collagen substratum. The addition of a mixture of bovine brain gangliosides (BBG) or the monosialoganglioside GM1 to this medium potentiated the nerve growth factor (NGF)-independent CM-mediated neuritogenesis. A 3-4 fold increase in spinal cord outgrowth was due to increased neurite number, length and branching. The ability of the gangliosides to potentiate the positive neuritogenic action of CM was not limited solely to spinal cord cultures since similar results were obtained in parallel studies employing organized cultures of embryonic chick ciliary ganglia. These studies demonstrate the ability of gangliosides to enhance the trophic action of factor(s) present in CM. They suggest further that gangliosides may play a modulatory role in the development of the nervous system. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
