2型糖尿病并大血管病变患者载脂蛋白B基因多态性研究

目的 研究载脂蛋白B（ApoB）基因变异与2型糖尿病大血管病变的关系。方法 对65例2型糖尿病合并大血管病变患者和60名对照组的 ApoB基因EcoRⅠ和XbaⅠ酶切位点限制性片段长度多态性（PCR－RFLP）进行研究。结果 2型糖尿病大血管病变组载脂蛋白B少见等位基因频率（X^ ,E^-）显著升高,与对照组比较,E^-等位基因频率改变与2型糖尿病患者血脂异常有相关性,X^ 等位基因频率升高与2型糖尿病并大血管病变明显相关。结论 遗传因素在2型糖尿病大血管病变中起重要作用。

Relationship between apoB gene polymorphism and type 2 diabetes mellitus with macroangiopathy

Objective To investigate the relationship between the apolipoprotein B(ApoB) gene polymorphic sites and macroangiopathy in type 2 diabetes mellitus.Methods Two polymorphic sites XbaI,EcoRI of ApoB gene of 65 patients and 60 mormal controls were detected by PCR and PCR-RFLP.Results (1)The frequency of rare allele (X+,E-)was lower in Chinese than that in Caucasian.(2)Patients who own rare allele X+ had higher TG,LDL-C, ApoB and lower HDL-C.(3)The type 2 diabetes mellitus with macroangiopathy had much higher frequency of rare allele(X+,E-).Conclusion X+ allele may play a role in macroangiopathy in type 2 diabetes mellitus.