HPLC法测定接骨丸中马钱子士的宁含量

目的:利用高效液相色谱法测定接骨丸中成药马钱子士的宁的含量,为控制接骨丸的质量提供可靠依据。方法:利用高效液相色谱法,waters C18(250 mm×4.6 mm,5μm)反相色谱柱;流动相∶乙腈∶0.2%磷酸水溶液(20∶70)等度洗脱;柱温:25℃;流速:1.0 m L·min-1;检测波长:260 nm。结果:士的宁的线性范围是0.080 g·L-1~0.400 g·L-1;r值均为0.99997,平均加样回收率为99.30%,RSD分别为0.89%。结论:利用高效液相色谱法测定接骨丸中士的宁的含量,具有良好的重现性和专属性,且准确快捷,能够很好地控制接骨丸的内在质量。

Content of Strychnine in Bone-Setting Pills Determined by HPLC

Objective:To determine the content of strychnine in Bone-Setting Pills by HPLC to provide reliable evidence for the controlling of the quality of Bone-Setting Pills.Methods:HPLC was applied with reversed phase column of waters C18 (250 mm×4.6 mm,5 μm);mobile phase∶ acetonitrile ∶0.2％ phosphoric acid solution (20 ∶ 70);column temperature:25 ℃;flow velocity:1.0 mL · min-1;detected wave length:260nm.Results:The linearity range of strychnine was 0.080g · L-1 to 0.400 g · L-1;rvalue was 0.999 97 in average;the average recovery rate was 99.30％;RSD was 0.89％.Conclusion:HPLC has good reproducibility,specificity,accuracy and efficiency in determining the content of strychnine in Bone-Setting Pills and controlling the qulity of Bone-Setting Pills.