柴芍乳癖颗粒对乳腺增生兔模型B超声像及组织形态学的影响

目的：观察柴芍乳癖颗粒对乳腺增生病兔模型乳腺组织B超声像及组织形态学的影响。方法56只健康未孕新西兰雌兔，随机挑出10只为正常对照组，肌注生理盐水；46只为模型制备组，序贯肌注雌孕激素造模。2组均随机处死1只雌兔，观察病理切片确定造模成功后，将模型制备组剩下的45只兔再随机分为5组：模型对照组、乳癖散结颗粒组、柴芍乳癖颗粒低、中、高剂量组。6组兔每天分别予以生理盐水、乳癖散结颗粒（0.56 g/kg）、柴芍乳癖颗粒（0.47、0.94、1.88 g/kg）水溶液灌胃1次，连续3月。末次灌胃24 h后麻醉，用B超机采集第2对乳房的声像图，处死后取第2对乳腺组织，HE染色观察组织形态学表现，计数乳腺小叶腺泡数、导管上皮细胞层数、腺泡腔直径。结果柴芍乳癖颗粒能使模型兔乳腺组织低回声区缩小，内部回声强度减低，其中高剂量组最明显；与模型组相比，柴芍乳癖颗粒低、中、高剂量跟乳癖散结颗粒均可明显缩小腺泡腔直径（P＜0.01），中、高剂量可以减少乳腺导管上皮细胞层数，低剂量还可减少乳腺小叶腺泡数（P＜0.05）。结论柴芍乳癖颗粒可以改善乳腺增生兔模型B超声像,减轻雌兔乳腺增生程度，治疗乳腺增生效果明显。

Effects of Chaishao Rupi Granules on B Type Ultrasound Images and Histomorphology of Rabbit Models with Breast Hyperplasia

〔Abstract〕 Objective To observe the effect of Chaishao Rupi Granules on B type ultrasound images and histomorphology of rabbit models with breast hyperplasia. Methods 10 rabbits were randomly selected from 56 healthy unfertilized female New Zealand rabbits as the normal control group, which were given muscle injection with normal saline, 46 rabbits as model preparation group, which were taken female progestogen sequential muscle injection. Randomly killing female rats in the two groups, determine the model is successful or not by observing the pathological section. The remaining 45 rabbits in model preparation group were divided into 5 groups:model control group, Rupi Sanjie granule group, and low dose, middle dose, high dose of Chaishao Rupi granules groups. The six groups were administrated normal saline, 0.56 g/kg Rupi Sanjie granules, and 0.47 g/kg, 0.94 g/kg, 1.88 g/kg Chaishao Rupi granules, respectively, once a day, continuous for 3 months. At 24 h after the last lavage, we implemented the anesthesia to all of the rabbits and collected the second pair of breasts' B ultrasound images. We cut the second pair of breast tissue out. The histomorphology after HE staining was observed. The numbers of lobular acini, duct epithelial layer and acinus cavity diameter were counted. Results Chaishao Rupi granules can shrink the rabbit model's breast tissue keyed acoustic area, decrease internal echo intensity, the effect of high dose group is the most obvious. Compared with model group, three doses of Chaihu Rupi granules and Rupi Sanjie granules can narrow the acinus cavity diameter significantly (P<0.01). The middle dose and high dose can reduce the mammary ductal epithelial cell layers(P<0.05), low dose can also reduce lobular acini number (P<0.05). Conclusion Chaishao Rupi granules can improve B ultrasonicimage of mammary gland hyperplasia rabbit models, can reduce the degree of female rabbits' hyperplasia of mammary glands. The effect on hyperplasia of mammary glands is obvious.