不同组织器官中华蛇根草绿原酸含量的比较研究

目的：比较中华蛇根草不同组织器官中绿原酸的含量。方法选用Inertsil C18色谱柱，以甲醇-0.02%磷酸溶液（20∶80）为流动相，流速为1.0 mL/min，检测波长为325 nm，柱温40℃。结果绿原酸在0.027~1.700μg(r=0.9999)范围内线性关系良好。2月花期采收的中华蛇根草茎、叶、花中绿原酸的含量分别为3.39、0.77、22.79 mg/g。结论中华蛇根草不同组织器官绿原酸含量差异较大，其中花含量最高。该方法能准确、快速地进行定性定量检测，可用于中华蛇根草中绿原酸含量的测定。

Comparative Study on the Content of Chlorogenic Acid in Ophiorrhiza chinensis from Different Organs

Objective To determine the content of chlorogenic acid in different organs of Ophiorrhiza chinensis. Methods The contents of chlorogenic acid was determined with chromatographic column Inertsil C 18, And its mobile phase was methanol: 0.02% phosphoric acid solution (20:80), the flow rate was 1.0 mL/min, detection wavelength was 325 nm and the column temperature was 40 ℃. Results There was a good linear relationship of chlorogenic acid between 0.027 and 1.700 μg (r=0.999 9). The content of chlorogenic acid of Japanese snakeroot which were harvested during February flowering period, in stems, leaves, flowers were 3.39, 0.77, 22.79 mg/g, respectively. Conclusion The contents of chlorogenic acid, which are also different in different organs of the Ophiorrhiza chinensis, the highest of which is the flower. The method is accurate and quick in qualitative identification and quantitative assay, which can be used for the quality control of Ophiorrhiza Chinensis.